Analytical Data
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Gene name
SH3BP5
- Application
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Alternative Names
3BP5_HUMAN; OTTHUMP00000208289; OTTHUMP00000208291; OTTHUMP00000208294; SAB; SH3 binding protein; SH3 domain binding protein 5 (BTK associated); SH3 domain binding protein 5; SH3 domain binding protein that preferentially associates with BTK; SH3 domain-binding protein 5; SH3 domain-binding protein that preferentially associates with BTK; SH3 domainbinding protein 5; SH3BP 5; SH3BP-5; Sh3bp5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60239
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Expression Region
1-455 aa
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AA Sequence
MDAALKRSRS EEPAEILPPA RDEEEEEEEG MEQGLEEEEE VDPRIQGELE KLNQSTDDIN RRETELEDAR QKFRSVLVEA TVKLDELVKK IGKAVEDSKP YWEARRVARQ AQLEAQKATQ DFQRATEVLR AAKETISLAE QRLLEDDKRQ FDSAWQEMLN HATQRVMEAE QTKTRSELVH KETAARYNAA MGRMRQLEKK LKRAINKSKP YFELKAKYYV QLEQLKKTVD DLQAKLTLAK GEYKMALKNL EMISDEIHER RRSSAMGPRG CGVGAEGSST SVEDLPGSKP EPDAISVASE AFEDDSCSNF VSEDDSETQS VSSFSSGPTS PSEMPDQFPA VVRPGSLDLP SPVSLSEFGM MFPVLGPRSE CSGASSPECE VERGDRAEGA ENKTSDKANN NRGLSSSSGS GGSSKSQSST SPEGQALENR MKQLSLQCSK GRDGIIADIK MVQIG
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Molecular Weight
50.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SH3BP5, or SH3 domain-binding protein 5, plays a crucial role in various cellular processes, including cell signaling, cytoskeletal organization, and endocytosis. Its interaction with SH3 domain-containing proteins suggests its involvement in mediating protein-protein interactions that are vital for cellular responses to external stimuli. Research has indicated that SH3BP5 may influence the dynamics of actin remodeling, contributing to processes such as cell migration and adhesion. Additionally, its dysregulation has been linked to several diseases, including cancer, where altered signaling pathways can lead to enhanced tumor growth and metastasis. The study of SH3BP5 as a recombinant protein provides insights into its functional roles and mechanisms via in vitro assays, facilitating the identification of potential therapeutic targets. Understanding the structural and functional properties of SH3BP5 could advance our knowledge of its biological significance and lead to novel strategies for disease intervention. By exploring the recombinant expression of SH3BP5, researchers aim to elucidate its specific interactions and pathways, thereby contributing to the broader understanding of cell biology and the pathological processes associated with its dysfunction.
