Analytical Data
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Gene name
Map3k8
- Application
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Alternative Names
Map3k8；COT；ESTF；Mitogen-activated Protein kinase kinase kinase 8
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P46527
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Expression Region
30-397aa
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AA Sequence
MENLYASEEP AVYEPSLMTM CQDSNQNDER SKSLLLSGQE VPWLSSVRYG TVEDLLAFAN HISNTAKHFY GQRPQESGIL LNMVITPQNG RYQIDSDVLL IPWKLTYRNI GSDFIPRGAF GKVYLAQDIK TKKRMACKLI PVDQFKPSDV EIQACFRHEN IAELYGAVLW GETVHLFMEA GEGGSVLEKL ESCGPMREFE IIWVTKHVLK GLDFLHSKKV IHHDIKPSNI VFMSTKAVLV DFGLSVQMTE DVYFPKDLRG TEIYMSPEVI LCRGHSTKAD IYSLGATLIH MQTGTPPWVK RYPRSAYPSY LYIIHKQAPP LEDIADDCSP GMRELIEASL ERNPNHRPRA ADLLKHEALN
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Molecular Weight
70 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Map3k8, also known as Mitogen-Activated Protein Kinase Kinase Kinase 8, is a crucial enzyme involved in various cellular signaling pathways, particularly in the regulation of stress responses and inflammatory processes. The protein plays a significant role in the activation of MAPK cascades, which are essential for mediating cellular responses to growth factors, cytokines, and environmental stresses. Research on Map3k8 has gained traction due to its implications in several diseases, including cancer, autoimmune disorders, and neurodegenerative conditions. Understanding the molecular mechanisms of Map3k8 not only sheds light on fundamental biological processes but also presents potential therapeutic targets for treating conditions where this kinase is dysregulated. Recent studies have focused on the characterization of recombinant Map3k8 to elucidate its structure-function relationship and to develop specific inhibitors that could modulate its activity. This research is critical for advancing our understanding of the therapeutic potential of targeting Map3k8 in various pathophysiological contexts, highlighting its relevance in modern biomedical research.
