Analytical Data
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Gene name
RHOT1
- Application
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Alternative Names
RHOT1；KIAA1042；OIP106；Trafficking kinesin-binding Protein 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IXI2
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Expression Region
1-681aa
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AA Sequence
MKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHISDGVADSGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFKVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR
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Molecular Weight
70.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RHOT1, a member of the Ras superfamily of GTPases, has garnered significant attention in recent years due to its pivotal role in cellular processes such as mitochondrial dynamics, intracellular transport, and cell signaling. It is specifically implicated in the regulation of mitochondrial morphology and function, making it a critical factor in cellular energy metabolism and apoptosis. Dysregulation of RHOT1 has been associated with various pathological conditions, including cancer and neurodegenerative diseases, suggesting its potential as a therapeutic target. Research on RHOT1 recombinant proteins has focused on elucidating its biochemical properties and understanding its interaction with downstream effectors, as well as its impact on mitochondrial integrity and cellular homeostasis. By employing recombinant DNA technology, researchers are able to produce RHOT1 proteins in sufficient quantities for functional studies, allowing for a deeper investigation into its molecular mechanisms. This has implications not only for basic biology but also for developing strategies to modulate RHOT1 activity in disease contexts. Overall, RHOT1 represents a promising area of study within the field of cellular biology and could provide new insights into the complex regulation of mitochondrial functions and their contributions to health and disease.
