Analytical Data
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基因名
COCH
- Application
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别名
COCH; COCH5B2; UNQ257/PRO294Cochlin; COCH-5B2
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43405
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表达区间
1-494aa
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氨基酸序列
MSAAWIPALGLGVCLLLLPGPAGSEGAAPIAITCFTRGLDIRKEKADVLCPGGCPLEEFSVYGNIVYASVSSICGAAVHRGVISNSGGPVRVYSLPGRENYSSVDANGIQSQMLSRWSASFTVTKGKSSTQEATGQAVSTAHPPTGKRLKKTPEKKTGNKDCKADIAFLIDGSFNIGQRRFNLQKNFVGKVALMLGIGTEGPHVGLVQASEHPKIEFYLKNFTSAKDVLFAIKEVGFRGGNSNTGKALKHTAQKFFTVDAGVRKGIPKVVVVFIDGWPSDDIEEAGIVAREFGVNVFIVSVAKPIPEELGMVQDVTFVDKAVCRNNGFFSYHMPNWFGTTKYVKPLVQKLCTHEQMMCSKTCYNSVNIAFLIDGSSSVGDSNFRLMLEFVSNIAKTFEISDIGAKIAAVQFTYDQRTEFSFTDYSTKENVLAVIRNIRYMSGGTATGDAISFTVRNVFGPIRESPNKNFLVIVTDGQSYDDVQGPAAAAHDAAK
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分子量
79.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cochlin (COCH) is a widely studied extracellular matrix protein known for its role in the development and maintenance of the inner ear and various connective tissues. Research has increasingly focused on COCH due to its implication in several auditory disorders, including non-syndromic hearing loss. Mutations in the COCH gene have been linked to these conditions, highlighting its significance in auditory function and integrity. The protein exhibits a unique structure that allows it to interact with other extracellular matrix components, influencing cellular behavior, tissue organization, and possibly inflammatory responses. Additionally, COCH's involvement in processes such as tissue repair and regeneration makes it a candidate for therapeutic approaches targeting hearing loss and other related conditions. Investigating the recombinant expression of COCH protein is essential to elucidate its biological functions, explore its potential as a biomarker for auditory diseases, and assess its viability as a therapeutic target. Through the production of recombinant COCH, researchers aim to study its biochemical properties, assess its interactions with other molecules, and investigate its role in cellular signaling pathways associated with hearing mechanisms. Understanding the complexities of COCH can pave the way for developing innovative strategies for diagnosing and treating hearing loss and other disorders linked to extracellular matrix dysfunction. As such, tailored recombinant COCH proteins could also serve as tools in the regenerative medicine field, potentially offering insights into novel therapeutic options for auditory and connective tissue-related diseases.












