Analytical Data
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Gene name
PDE1C
- Application
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Alternative Names
PDE1C；Dual specificity calcium/calmodulin-dependent 3'.5'-cyclic nucleotide phosphodiesterase 1C
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14123-2
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Expression Region
2-634aa
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AA Sequence
ESPTKEIEEFESNSLKYLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQL ERGEASVVDLKKNLEYAATVLESVYIDETRRLLDTEDELSDIQSDAVPSE VRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFVERMYRRTSNMV GLSYPPAVIEALKDVDKWSFDVFSLNEASGDHALKFIFYELLTRYDLISR FKIPISALVSFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVAN WLTELEIFAIIFSAAIHDYEHTGTTNNFHIQTRSDPAILYNDRSVLENHH LSAAYRLLQDDEEMNILINLSKDDWREFRTLVIEMVMATDMSCHFQQIKA MKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQ GDREAELGLPFSPLCDRKSTMVAQSQVGFIDFIVEPTFTVLTDMTEKIVS PLIDETSQTGGTGQRRSSLNSISSSDAKRSGVKTSGSEGSAPINNSVISV DYKSFKATWTEVVHINRERWRAKVPKEEKAKKEAEEKARLAAEEQQKEME AKSQAEEGASGKAEKKTSGETKNQVNGTRANKSDNPRGKNSKAEKSSGEQ QQNGDFKDGKNKTDKKDHSNIGNDSKKTDDSQE
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Molecular Weight
99 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PDE1C (Phosphodiesterase 1C) is an important enzyme that plays a critical role in the hydrolysis of cyclic nucleotides, specifically cAMP and cGMP, thereby regulating various physiological processes, including neuronal signaling, muscle contraction, and vascular function. The study of PDE1C is particularly relevant in the context of cardiovascular diseases and neurological disorders, where dysregulation of cyclic nucleotide signaling is often implicated. Recent advancements in biotechnology have enabled the production of recombinant PDE1C proteins, facilitating detailed structural and functional studies of this enzyme. Understanding the catalytic mechanisms and regulatory properties of PDE1C at the molecular level can provide insights into its role in health and disease. Moreover, PDE1C has emerged as a potential pharmacological target for drug development, as selective inhibitors could offer therapeutic strategies for treating conditions like heart failure and cognitive dysfunction. Consequently, researchers are focused on characterizing recombinant PDE1C to explore its interactions with substrates and inhibitors, paving the way for innovative treatments that could enhance therapeutic outcomes by modulating cyclic nucleotide levels in target tissues. The development of PDE1C recombinant proteins is thus a significant step towards elucidating the enzyme's biological functions and its implications in disease mechanisms.
