Analytical Data
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Gene name
POU4F2
- Application
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Alternative Names
POU4F2；BRN3B；POU domain. class 4. transcription factor 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q12837
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Expression Region
1-265aa
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AA Sequence
MNTIPCTSAASSSSVPISHPSALAGTHHHHHHHHHHHHQPHQALEGELLEHLSPGLALGAMAGPDGAVVSTPAHAPHMATMNPMHQAALSMAHAHGLPSHMGCMSDVDADPRDLEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEKSHREKLTKPELFNGAEKKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAGI
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Molecular Weight
36.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
POU4F2, also known as Brn-3b, is a member of the POU (Pit-Oct-Unc) transcription factor family, which plays a crucial role in the development and differentiation of sensory neurons, particularly in the visual and auditory systems. Research into POU4F2 has gained prominence due to its involvement in the regulation of gene expression in neurons and its potential implications in neurodevelopmental disorders and sensory deficits. The protein is known to be essential for the survival of retinal ganglion cells and other related neuronal populations. Abnormal expression or mutations in POU4F2 have been linked to various pathologies, including pseudotumor cerebri and other conditions affecting sensory perception. The study of recombinant POU4F2 proteins has been pivotal in delineating its function, as these proteins can be used to explore interaction with target genes and proteins, thus helping to unravel the intricate signaling pathways governing neuronal development. Moreover, the generation of POU4F2 recombinants facilitates the investigation of therapeutic interventions aiming to enhance neuronal survival and function, particularly in degenerative diseases where sensory neuron integrity is compromised. Understanding the mechanistic roles of POU4F2 not only sheds light on fundamental neurobiological processes but also informs the development of potential strategies for treating disorders linked to its dysregulation. Overall, the exploration of POU4F2 recombinant protein remains a significant avenue for advancing our comprehension of sensory neuron biology and its associated clinical ramifications.
