Analytical Data
-
基因名
MED1
- Application
-
别名
MED1；ARC205；CRSP1；CRSP200；Mediator of RNA polymerase II transcription subunit 1
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q15648
-
表达区间
878-1031aa
-
氨基酸序列
FGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPF
-
分子量
32.2 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MED1, part of the mediator complex, plays a crucial role in the regulation of gene expression by serving as a bridge between transcription factors and the RNA polymerase II machinery. Recent studies have highlighted the significance of MED1 in various biological processes, including cell differentiation, development, and maintenance of stem cell pluripotency. Aberrant expression or function of MED1 has been linked to several diseases, particularly cancer, where it can influence tumor growth and metastasis through the regulation of key oncogenic pathways. Understanding the structure and function of MED1 at the molecular level can provide insights into its regulatory mechanisms and potential as a therapeutic target. Researchers have focused on the recombinant expression of MED1 protein to facilitate structural studies and functional assays, contributing to the elucidation of its role in mediating transcriptional responses. The development of MED1-based interventions holds promise for innovative therapeutic strategies aimed at modulating gene expression in disease contexts, underscoring the importance of continued research in this area.
