Analytical Data
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基因名
NQO1
- Application
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别名
NQO1；DIA4；NMOR1；NAD(P)H dehydrogenase [quinone] 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P15559
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表达区间
2-274aa
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氨基酸序列
VGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NQO1 (NAD(P)H:quinone oxidoreductase 1) is an essential enzyme involved in the cellular defense mechanism against oxidative stress, playing a crucial role in the detoxification of electrophilic compounds and the protection of cells from damage caused by reactive oxygen species. Research has shown that NQO1 is crucial for maintaining cellular redox balance and has implications in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Variants of the NQO1 gene have been linked to altered enzyme activity, influencing individual susceptibility to toxicants and the efficacy of chemotherapies. As a result, recombinant NQO1 protein has gained attention for its potential applications in drug development, biomarker discovery, and therapeutic interventions. The study of NQO1 involves various techniques, including gene cloning, protein expression systems, and purification methods, aiming to elucidate its structure-function relationship and regulatory mechanisms. Understanding the role of NQO1 in health and disease not only enhances our comprehension of oxidative stress management but also opens avenues for developing targeted therapies that could improve patient outcomes in conditions where NQO1 activity is compromised.
