Analytical Data
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Gene name
PAPPA2
- Application
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Alternative Names
PAPPA2；PLAC3；Pappalysin-2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BXP8-1
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Expression Region
234-1396aa
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AA Sequence
SPPEESNQNG GEGSYREAET FNSQVGLPIL YFSGRRERLL LRPEVLAEIP REAFTVEAWV KPEGGQNNPA IIAGVFDNCS HTVSDKGWAL GIRSGKDKGK RDARFFFSLC TDRVKKATIL ISHSRYQPGT WTHVAATYDG RHMALYVDGT QVASSLDQSG PLNSPFMASC RSLLLGGDSS EDGHYFRGHL GTLVFWSTAL PQSHFQHSSQ HSSGEEEATD LVLTASFEPV NTEWVPFRDE KYPRLEVLQG FEPEPEILSP LQPPLCGQTV CDNVELISQY NGYWPLRGEK VIRYQVVNIC DDEGLNPIVS EEQIRLQHEA LNEAFSRYNI SWQLSVHQVH NSTLRHRVVL VNCEPSKIGN DHCDPECEHP LTGYDGGDCR LQGRCYSWNR RDGLCHVECN NMLNDFDDGD CCDPQVADVR KTCFDPDSPK RAYMSVKELK EALQLNSTHF LNIYFASSVR EDLAGAATWP WDKDAVTHLG GIVLSPAYYG MPGHTDTMIH EVGHVLGLYH VFKGVSERES CNDPCKETVP SMETGDLCAD TAPTPKSELC REPEPTSDTC GFTRFPGAPF TNYMSYTDDN CTDNFTPNQV ARMHCYLDLV YQQWTESRKP TPIPIPPMVI GQTNKSLTIH WLPPISGVVY DRASGSLCGA CTEDGTFRQY VHTASSRRVC DSSGYWTPEE AVGPPDVDQP CEPSLQAWSP EVHLYHMNMT VPCPTEGCSL ELLFQHPVQA DTLTLWVTSF FMESSQVLFD TEILLENKES VHLGPLDTFC DIPLTIKLHV DGKVSGVKVY TFDERIEIDA ALLTSQPHSP LCSGCRPVRY QVLRDPPFAS GLPVVVTHSH RKFTDVEVTP GQMYQYQVLA EAGGELGEAS PPLNHIHGAP YCGDGKVSER LGEECDDGDL VSGDGCSKVC ELEEGFNCVG EPSLCYMYEG DGICEPFERK TSIVDCGIYT PKGYLDQWAT RAYSSHEDKK KCPVSLVTGE PHSLICTSYH PDLPNHRPLT GWFPCVASEN ETQDDRSEQP EGSLKKEDEV WLKVCFNRPG EARAIFIFLT TDGLVPGEHQ QPTVTLYLTD VRGSNHSLGT YGLSCQHNPL IINVTHHQNV LFHHTTSVLL NFSSPRVGIS AVALRTSSRI GLSAPSNCIS EDEGQNHQGQ SCIHRPCGKQ DSC
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Molecular Weight
131 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PAPPA2 (Pregnancy-Associated Plasma Protein A2) is a metalloproteinase enzyme that plays a vital role in the regulation of insulin-like growth factor (IGF) signaling pathways. It is involved in the modulation of IGF availability, thereby influencing cellular growth, differentiation, and metabolic processes. Recent studies have highlighted its significance in various physiological states, including pregnancy and cancer, where its expression levels can be altered. The recombinant production of PAPPA2 protein has garnered interest for its potential applications in both therapeutic and diagnostic contexts. By producing PAPPA2 in a controlled laboratory setting, researchers aim to further investigate its biochemical properties, interactions with IGF-binding proteins, and its overall role in disease mechanisms. Furthermore, the recombinant protein may serve as a valuable tool for understanding the complex regulatory networks involved in growth and development, as well as for exploring innovative treatments for conditions characterized by dysregulated IGF signaling. The study of PAPPA2 and its recombinant forms not only contributes to the foundational understanding of growth regulation but also holds promise for translational applications in medicine and biotechnology.
