Analytical Data
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基因名
CHC1
- Application
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别名
RCC1. CHC1 Ran GEF; RanGEF; RCC 1; RCC1; RCC1 I; RCC1_HUMAN
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P18754
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表达区间
2-421
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氨基酸序列
SPKRIAKRR SPPADAIPKS KKVKVSHRSH STEPGLVLTL GQGDVGQLGL GENVMERKKP ALVSIPEDVV QAEAGGMHTV CLSKSGQVYS FGCNDEGALG RDTSVEGSEM VPGKVELQEK VVQVSAGDSH TAALTDDGRV FLWGSFRDNN GVIGLLEPMK KSMVPVQVQL DVPVVKVASG NDHLVMLTAD GDLYTLGCGE QGQLGRVPEL FANRGGRQGL ERLLVPKCVM LKSRGSRGHV RFQDAFCGAY FTFAISHEGH VYGFGLSNYH QLGTPGTESC FIPQNLTSFK NSTKSWVGFS GGQHHTVCMD SEGKAYSLGR AEYGRLGLGE GAEEKSIPTL ISRLPAVSSV ACGASVGYAV TKDGRVFAWG MGTNYQLGTG QDEDAWSPVE MMGKQLENRV VLSVSSGGQH TVLLVKDKEQ S
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分子量
44.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHC1, or clathrin heavy chain 1, is a crucial component of the clathrin-mediated endocytosis pathway, which plays a vital role in cellular transport and signal transduction by facilitating the internalization of various molecules, including receptors and nutrients. The study of CHC1 recombinant proteins has gained significant attention due to their potential implications in understanding intracellular processes and their relevance in various diseases, including cancer and neurodegenerative disorders. Researchers have increasingly focused on the structural and functional characterization of CHC1 to elucidate its role in clathrin coat formation and the subsequent endocytic events. Furthermore, the ability to produce CHC1 as a recombinant protein allows for the investigation of its interactions with other cellular proteins and lipids, enabling the exploration of therapeutic targets that could modulate endocytic pathways. The insights gained from CHC1 research may hold promise for the development of novel strategies aimed at manipulating endocytosis for therapeutic benefit, emphasizing the importance of this protein in both basic biology and clinical applications.
