Analytical Data
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Gene name
PLA2G2A
- Application
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Alternative Names
PLA2G2A；PLA2B；PLA2L；RASF-A；Phospholipase A2. membrane associated
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P14555
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Expression Region
21-144aa
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AA Sequence
NLVNFHRMIKLTTGKEAALSYGFYGCHCGVGGRGSPKDATDRCCVTHDCCYKRLEKRGCGTKFLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKYQYYSNKHCRGSTPRC
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Molecular Weight
17.9kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PLA2G2A, or Secretory Phospholipase A2 Group IIA, is an important enzyme implicated in various physiological and pathological processes, including inflammation, host defense, and lipid metabolism. As a secreted enzyme, PLA2G2A plays a crucial role in the hydrolysis of phospholipids, leading to the release of lysophospholipids and free fatty acids, which can modulate cellular signaling and immune responses. Research has demonstrated that PLA2G2A is involved in the inflammatory response, making it a potential biomarker and therapeutic target for inflammatory diseases, such as rheumatoid arthritis and atherosclerosis. Furthermore, studies have indicated that PLA2G2A exhibits antimicrobial properties, suggesting its role in innate immunity against bacterial infections. The recombinant expression of PLA2G2A has gained attention for its potential applications in drug development and therapeutic strategies. By producing recombinant PLA2G2A in suitable expression systems, researchers can obtain large quantities of the enzyme for biochemical studies, structural characterization, and high-throughput screening of small molecules that may modulate its activity. Understanding the structure-function relationship of PLA2G2A through recombinant technology can provide insights into its mechanism of action and facilitate the development of novel inhibitors or modulators. Given the enzyme's significance in health and disease, the study of PLA2G2A as a recombinant protein is a promising area of research that may lead to advancements in therapeutic interventions and diagnostic tools for various inflammatory and infectious diseases.
