Analytical Data
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Gene name
Cap
- Application
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Alternative Names
Cap；CAP；Adenylyl cyclase-associated Protein 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P35237
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Expression Region
1-376aa
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AA Sequence
MDVLAEANGT FALNLLKTLG KDNSKNVFFS PMSMSCALAM VYMGAKGNTA AQMAQILSFN KSGGGGDIHQ GFQSLLTEVN KTGTQYLLRM ANRLFGEKSC DFLSSFRDSC QKFYQAEMEE LDFISAVEKS RKHINTWVAE KTEGKIAELL SPGSVDPLTR LVLVNAVYFR GNWDEQFDKE NTEERLFKVS KNEEKPVQMM FKQSTFKKTY IGEIFTQILV LPYVGKELNM IIMLPDETTD LRTVEKELTY EKFVEWTRLD MMDEEEVEVS LPRFKLEESY DMESVLRNLG MTDAFELGKA DFSGMSQTDL SLSKVVHKSF VEVNEEGTEA AAATAAIMMM RCARFVPRFC ADHPFLFFIQ HSKTNGILFC GRFSSP
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Molecular Weight
43 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cap proteins, or cap-binding proteins, play a crucial role in the regulation of gene expression and mRNA stability in eukaryotic cells. They are integral to the formation of the mRNA cap structure, a modified guanine nucleotide added to the 5' end of eukaryotic mRNA molecules during transcription. This cap is essential for mRNA stability, efficient translation, and the proper processing of RNA. In the context of cellular processes, the Cap protein facilitates mRNA transport from the nucleus to the cytoplasm and protects mRNA from degradation by exonucleases. Given their significant roles, abnormalities in Cap protein function can lead to various diseases, including cancer and neurodegenerative disorders. Consequently, research on Cap proteins has focused on understanding their molecular mechanisms, interactions with other cellular factors, and their involvement in the post-transcriptional regulation of gene expression. Recent advancements in structural biology and molecular techniques have enabled researchers to elucidate the intricate dynamics of Cap proteins, thus paving the way for potential therapeutic applications targeting these pathways. The study of Cap protein re-assembly and its implications in cellular physiology and pathology remains a significant field of research, aiming to uncover novel insights into gene regulation and the broader implications for human health.
