Analytical Data
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Gene name
melA
- Application
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Alternative Names
melA；Aspulvinone E synthetase melA
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O34645
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Expression Region
1-432aa
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AA Sequence
MKKITFIGAGSTIFAKNVLGDCLLTEALNGFEFALYDIDPKRLQESQLMLENLRDRYNPSVAINSYDDRKLALQNAGYVINAIQVGGYKPSTVIDFEIPKRYGLRQTIADTVGIGGIFRSLRTIPVLFDIAKDMEEMCPDAWFLNYTNPMATLTGAMLRYTNIKTIGLCHSVQVCTKDLFKALGMEHDGIEERIAGINHMAWLLEVKKDGTDLYPEIKRRAKEKQKTKHHDMVRFELMDKFGYYVTESSEHNAEYHPYFIKRNYPELISELQIPLDEYPRRCVKQIENWEKMRDDIVNNKNLTHERSKEYGSRIIEAMETNEPFTFGGNVLNTGLITNLPSKAVVEVTCVADRKKITPCFAGELPEQLAALNRTNINTQLMTIEAAVTRKKEAVYQAAMLDPHTSAELSMKDIISMCDDLFAAHGDWLPEYK
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Molecular Weight
51.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of melA recombinant protein is situated at the intersection of molecular biology and biotechnology, particularly in the context of microbial metabolism and enzyme engineering. MelA encodes for an enzyme known as alpha-melibiase, which is involved in the degradation of melibiose, a disaccharide sugar found in certain plant materials. Understanding the structure and function of melA is crucial for comprehending the metabolic pathways utilized by various microorganisms, especially those in the gut microbiome of humans and other animals. The significance of melA extends beyond basic biology; by producing recombinant melA protein, researchers can explore its potential applications in food biotechnology, such as in the fermentation industry, where it could facilitate the breakdown of sugars into useful by-products. Additionally, the study of melA can contribute to bioremediation efforts, where engineered enzymes are employed to degrade environmental pollutants. As researchers harness genetic engineering techniques, the recombinant melA protein offers insights not only into enzyme function and efficiency but also into broader metabolic interactions, paving the way for advances in sustainable bioprocesses and therapeutic interventions. Overall, the investigation of melA and its recombinant forms presents a valuable opportunity to enhance our understanding of microbial enzymology while also exploring practical applications in various industrial sectors.
