Analytical Data
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基因名
RUSC1
- Application
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别名
RUSC1; NESCARUN and SH3 domain-containing protein 1; New molecule containing SH3 at the carboxy-terminus; Nesca
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BVN2
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表达区间
1-433 aa
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氨基酸序列
MAEAQSGTGQLQEQKKGLLIAVSVSVDKIISHFGAARNLVQKAQLGDSRLSPDVGHLVLTTLCPALHALVADGLKPFRKDLITGQRRSSPWSVVEASVKPGSSTRSLGTLYSQVSRLAPLSSSRSRFHAFILGLLNTKQLELWFSSLQEDAGLLSLLYLPTGFFSLARGGCPSLSTELLLLLQPLSVLTFHLDLLFEHHHHLPLGPPQAPAPPGPPPALQQTMQAMLHFGGRLAQSLRGTSKEAASDPSDSPNLPTPGSWWEQLTQASRVYASGGTEGFPLSRWAPGRHGTAAEEGAQERPLPTDEMAPGRGLWLGRLFGVPGGPAENENGALKSRRPSSWLPPTVSVLALVKRGAPPEMPSPQELEASAPRMVQTHRAVRALCDHTAARPDQLSFRRGEVLRVITTVDEDWLRCGRDGMEGLVPVGYTSLVL
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分子量
73 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RUSC1, also known as Rho GTPase-activating protein 1, is a critical regulator of Rho GTPases, which are pivotal in various cellular processes, including cytoskeletal dynamics, cell migration, and proliferation. The protein functions by promoting the conversion of Rho GTPases from their active GTP-bound form to the inactive GDP-bound form, thereby influencing signal transduction pathways. Research on RUSC1 has garnered interest due to its potential implications in cancer, where dysregulation of Rho GTPase activity is often linked to tumor progression and metastasis. Moreover, RUSC1's involvement in modulating cellular responses to stress and its possible interplay with other signaling molecules make it a candidate for therapeutic targeting. Studies have shown that RUSC1 expression levels can correlate with disease severity, highlighting its role as a potential biomarker. The cloning, expression, and purification of recombinant RUSC1 serve as a foundation for further functional studies aimed at elucidating its mechanisms in cellular signaling and its interactions with other proteins. Understanding the structure-function relationships of RUSC1 can pave the way for developing novel therapeutic strategies directed at manipulating Rho GTPase signaling in various diseases. Thus, continued investigation into RUSC1's role within the cellular context remains critical for expanding our knowledge of its contribution to cellular physiology and pathology.
