Analytical Data
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Gene name
RSPH1
- Application
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Alternative Names
RSPH1; TSA2; TSGA2; Radial spoke head 1 homolog; Cancer/testis antigen 79; CT79; Male meiotic metaphase chromosome-associated acidic protein; Meichroacidin; Testis-specific gene A2 protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8WYR4
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Expression Region
1-309 aa
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AA Sequence
MSDLGSEELE EEGENDIGEY EGGRNEAGER HGRGRARLPN GDTYEGSYEF GKRHGQGIYK FKNGARYIGE YVRNKKHGQG TFIYPDGSRY EGEWANDLRH GHGVYYYINN DTYTGEWFAH QRHGQGTYLY AETGSKYVGT WVNGQQEGTA ELIHLNHRYQ GKFLNKNPVG PGKYVFDVGC EQHGEYRLTD MERGEEEEEE ELVTVVPKWK ATQITELALW TPTLPKKPTS TDGPGQDAPG AESAGEPGEE AQALLEGFEG EMDMRPGDED ADVLREESRE YDQEEFRYDM DEGNINSEEE ETRQSDLQD
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Molecular Weight
35.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RSPH1 (Radial spoke head 1) is a gene that encodes a protein essential for the assembly and function of cilia and flagella, structures critical for cellular motility and signaling. Mutations in RSPH1 are associated with various genetic disorders, including primary ciliary dyskinesia (PCD), a condition characterized by impaired movement of cilia, leading to respiratory problems and infertility. The importance of RSPH1 in ciliary function has prompted researchers to investigate its role in the pathogenesis of related diseases. Recombinant RSPH1 protein offers a unique opportunity to elucidate the molecular mechanisms underlying ciliary structure and function. By producing this protein in a controlled laboratory environment, scientists can study its interactions with other proteins, assess its stability, and explore its functionality in cellular models. These insights are crucial for developing therapeutic strategies to address conditions arising from RSPH1 mutations. Additionally, recombinant RSPH1 can serve as a valuable tool in high-throughput screening assays aimed at identifying small molecules or compounds that can rescue the defective ciliary function associated with mutations in this gene. Understanding RSPH1’s role at the molecular level could pave the way for novel interventions, significantly enhancing our comprehension of ciliary biology and the associated diseases.
