Analytical Data
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基因名
alkB
- Application
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别名
alkB；ABH6；Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P05050
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表达区间
1-216aa
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氨基酸序列
MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQSPFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQRAATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTIDCRYNLTFRQAGKKE
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分子量
44.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AlkB, a member of the α-ketoglutarate-dependent dioxygenase family, plays a crucial role in the repair of damaged DNA through its oxidative demethylation activity. This enzyme has garnered significant interest due to its ability to reverse alkylation damage—specifically targeting methyl groups on DNA bases, which can result from environmental toxins and certain chemotherapeutic agents. The accumulation of such lesions is linked to various diseases, including cancer, making AlkB an attractive target for therapeutic research. Additionally, understanding the structure-function relationship of AlkB can provide insights into its catalytic mechanism and specificity. The recombinant expression of AlkB in various host systems, such as E. coli, allows for the production of large quantities of the protein for biochemical studies and structural analysis. This research can not only enhance our fundamental understanding of DNA repair mechanisms but also pave the way for the development of novel strategies to enhance DNA repair in therapeutic contexts, highlighting the significance of AlkB in both basic and applied biological research.
