Analytical Data
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基因名
CDC42EP4
- Application
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别名
CDC42EP4; BORG4; CEP4; Cdc42 effector Protein 4; Binder of Rho GTPases 4
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H3Q1
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表达区间
1-356aa
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氨基酸序列
MPILKQLVSSSVHSKRRSRADLTAEMISAPLGDFRHTMHVGRAGDAFGDTSFLNSKAGEPDGESLDEQPSSSSSKRSLLSRKFRGSKRSQSVTRGEREQRDMLGSLRDSALFVKNAMSLPQLNEKEAAEKGTSKLPKSLSSSPVKKANDGEGGDEEAGTEEAVPRRNGAAGPHSPDPLLDEQAFGDLTDLPVVPKATYGLKHAESIMSFHIDLGPSMLGDVLSIMDKEEWDPEEGEGGYHGDEGAAGTITQAPPYAVAAPPLARQEGKAGPDLPSLPSHALEDEGWAAAAPSPGSARSMGSHTTRDSSSLSSCTSGILEERSPAFRGPDRARAAVSRQPDKEFSFMDEEEEDEIRV
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分子量
64.4 KDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CDC42EP4, or Cell Division Cycle 42 Effector Protein 4, is a member of the CDC42 effector protein family and is known to play a critical role in various cellular processes, including cytoskeletal dynamics, cell migration, and signal transduction pathways. This protein interacts with the small GTPase CDC42, which is pivotal in regulating the actin cytoskeleton and influencing cell polarization and movement. Research has indicated that CDC42EP4 is involved in the regulation of cell proliferation and differentiation, suggesting its potential impact on developmental biology and cancer progression. Previous studies have identified its involvement in processes such as endocytosis and exocytosis, which are essential for cellular communication and nutrient uptake. Given its significant roles, the study of CDC42EP4's structure and function through recombinant protein techniques has become increasingly relevant. Researchers aim to elucidate the molecular mechanisms underlying CDC42EP4's interactions with other proteins and its impact on cellular mechanisms. By producing recombinant CDC42EP4, scientists can investigate its biophysical properties, binding affinities, and functional impacts in vitro, contributing to a better understanding of its biological relevance. These insights may pave the way for novel therapeutic approaches targeting CDC42EP4-related pathways in diseases characterized by aberrant cell migration and growth, such as cancer metastasis. As such, CDC42EP4 represents a promising target for further research, and the generation of its recombinant protein is a crucial step towards unraveling its multifaceted roles in cell biology.












