Cat: PA2000-2901

Recombinant Chicken VTG2 Protein,His

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Analytical Data

  • Gene name

    VTG2

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    VTG2;VTGII;Vitellogenin-2

  • Species

    Chicken

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P02845

  • Expression Region

    26-653aa

  • AA Sequence

    SRRSYLYNYEGSMLNGLQDRSLGKAGVRLSSKLEISGLPENAYLLKVRSPQVEEYNGVWPRDPFTRSSKITQVISSCFTRLFKFEYSSGRIGNIYAPEDCPDLCVNIVRGILNMFQMTIKKSQNVYELQEAGIGGICHARYVIQEDRKNSRIYVTRTVDLNNCQEKVQKSIGMAYIYPCPVDVMKERLTKGTTAFSYKLKQSDSGTLITDVSSRQVYQISPFNEPTGVAVMEARQQLTLVEVRSERGSAPDVPMQNYGSLRYRFPAVLPQMPLQLIKTKNPEQRIVETLQHIVLNNQQDFHDDVSYRFLEVVQLCRIANADNLESIWRQVSDKPRYRRWLLSAVSASGTTETLKFLKNRIRNDDLNYIQTLLTVSLTLHLLQADEHTLPIAADLMTSSRIQKNPVLQQVACLGYSSVVNRYCSQTSACPKEALQPIHDLADEAISRGREDKMKLALKCIGNMGEPASLKRILKFLPISSSSAADIPVHIQIDAITALKKIAWKDPKTVQGYLIQILADQSLPPEVRMMACAVIFETRPALALITTIANVAMKESNMQVASFVYSHMKSLSKSRLPFMYNISSACNIALKLLSPKLDSMSYRYSKVIRADTYFDNYRVGATGEIFVVNS

  • Molecular Weight

    74.9kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

VTG2, or vitellogenin 2, is a lipoglycoprotein primarily recognized for its crucial role in oogenesis and embryonic development in oviparous organisms. Research on VTG2 has gained considerable attention due to its implications in reproductive biology and its potential applications in biomonitoring environmental pollution. VTG2 is synthesized in the liver and transported to the ovaries, where it is incorporated into the developing oocytes, serving as a major source of nutrients for the growing embryos. Studies have shown that VTG2 levels can be significantly influenced by various environmental factors, including pollutants, which can disrupt endocrine functions and affect reproductive health. As such, VTG2 has emerged as a valuable biomarker for assessing the impact of pollutants in aquatic ecosystems. Furthermore, understanding the molecular mechanisms regulating VTG2 expression can provide insights into reproductive physiology and the evolutionary adaptability of species in response to environmental stresses. As a result, VTG2 is not only a key element in the study of fish and amphibian reproductive strategies but also serves as a vital indicator of ecosystem health, making it a significant target for ongoing research in developmental biology and environmental sciences.

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