Analytical Data
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Gene name
GPR114
- Application
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Alternative Names
GPR114；GPR114；PGR27；Adhesion G-Protein coupled receptor G5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IZF4
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Expression Region
1-184aa
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AA Sequence
MDHCGALFLC LCLLTLQNAT TETWEELLSY MENMQVSRGR SSVFSSRQLH QLEQMLLNTS FPGYNLTLQT PTIQSLAFKL SCDFSGLSLT SATLKRVPQA GGQHARGQHA MQFPAELTRD ACKTRPRELR LICIYFSNTH FFKDENNSSL LNNYVLGAQL SHGHVNNLRD PVNISFWHNQ SLEG
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Molecular Weight
46 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GPR114, also known as the G protein-coupled receptor 114, is an essential member of the G protein-coupled receptor (GPCR) family, which plays a critical role in various physiological processes and signaling pathways. Recent research has highlighted its involvement in diverse biological functions, including immune response, cell proliferation, and migration, making it a significant target for therapeutic interventions. The recombinant expression of GPR114 protein is crucial for understanding its structure, function, and the molecular mechanisms underlying its role in disease contexts, such as cancer and inflammatory disorders. Advances in recombinant protein technology have enabled the production of GPR114 in various expression systems, facilitating detailed biophysical characterization and functional assays. By studying GPR114, researchers aim to elucidate its signaling pathways and interactions with ligands, which can unveil potential drug targets. Additionally, GPR114's expression patterns in different tissues and its regulatory mechanisms further emphasize its biological relevance. Understanding GPR114 at the molecular level not only contributes to the basic knowledge of GPCR biology but also opens avenues for developing novel therapeutic strategies aimed at diseases associated with this receptor. Thus, the research on GPR114 recombinant protein stands at the intersection of basic science and translational medicine, highlighting its promise in drug discovery and development.
