Analytical Data
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基因名
GPR114
- Application
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别名
GPR114；GPR114；PGR27；Adhesion G-Protein coupled receptor G5
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8IZF4
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表达区间
1-184aa
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氨基酸序列
MDHCGALFLC LCLLTLQNAT TETWEELLSY MENMQVSRGR SSVFSSRQLH QLEQMLLNTS FPGYNLTLQT PTIQSLAFKL SCDFSGLSLT SATLKRVPQA GGQHARGQHA MQFPAELTRD ACKTRPRELR LICIYFSNTH FFKDENNSSL LNNYVLGAQL SHGHVNNLRD PVNISFWHNQ SLEG
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分子量
46 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GPR114, also known as the G protein-coupled receptor 114, is an essential member of the G protein-coupled receptor (GPCR) family, which plays a critical role in various physiological processes and signaling pathways. Recent research has highlighted its involvement in diverse biological functions, including immune response, cell proliferation, and migration, making it a significant target for therapeutic interventions. The recombinant expression of GPR114 protein is crucial for understanding its structure, function, and the molecular mechanisms underlying its role in disease contexts, such as cancer and inflammatory disorders. Advances in recombinant protein technology have enabled the production of GPR114 in various expression systems, facilitating detailed biophysical characterization and functional assays. By studying GPR114, researchers aim to elucidate its signaling pathways and interactions with ligands, which can unveil potential drug targets. Additionally, GPR114's expression patterns in different tissues and its regulatory mechanisms further emphasize its biological relevance. Understanding GPR114 at the molecular level not only contributes to the basic knowledge of GPCR biology but also opens avenues for developing novel therapeutic strategies aimed at diseases associated with this receptor. Thus, the research on GPR114 recombinant protein stands at the intersection of basic science and translational medicine, highlighting its promise in drug discovery and development.
