Analytical Data
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Gene name
RPP40
- Application
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Alternative Names
EC 3.1.26.5; Ribonuclease P protein subunit p40; RNase P subunit 1; RNaseP protein p40; RNASEP1; RPP40; RPP40_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75818
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Expression Region
1-363 aa
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AA Sequence
MATLRRLREA PRHLLVCEKS NFGNHKSRHR HLVQTHYYNY RVSFLIPECG ILSEELKNLV MNTGPYYFVK NLPLHELITP EFISTFIKKG SCYALTYNTH IDEDNTVALL PNGKLILSLD KDTYEETGLQ GHPSQFSGRK IMKFIVSIDL MELSLNLDSK KYERISWSFK EKKPLKFDFL LAWHKTGSEE STMMSYFSKY QIQEHQPKVA LSTLRDLQCP VLQSSELEGT PEVSCRALEL FDWLGAVFSN VDLNNEPNNF ISTYCCPEPS TVVAKAYLCT ITGFILPEKI CLLLEHLCHY FDEPKLAPWV TLSVQGFADS PVSWEKNEHG FRKGGEHLYN FVIFNNQDYW LQMAVGANDH CPP
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Molecular Weight
41.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RPP40, also known as ribonuclease P protein subunit p40, plays a crucial role in the processing of precursor tRNA, a fundamental step in the maturation of transfer RNA molecules within the cell. As a component of the ribonuclease P complex, RPP40 contributes to the enzymatic activity that cleaves RNA substrates, thus facilitating the synthesis of functional tRNA, which is essential for protein translation. Research into RPP40 has gained momentum due to its implications in various biological processes and potential associations with diseases. The importance of proper tRNA maturation has been linked to cellular stress responses, differentiation, and growth regulation, while dysregulation of RNA processing enzymes, such as those involving RPP40, has been observed in certain cancers and genetic disorders. Given the structural and functional complexity of the ribonuclease P complex, reconstituting RPP40 in a recombinant form proves essential for elucidating its biochemical properties and interactions. Studies employing recombinant RPP40 can enhance our understanding of its role in RNA biology, paving the way for potential therapeutic interventions that target RNA processing pathways. Furthermore, the development of RPP40 as a recombinant protein opens avenues for high-throughput screening methods aimed at identifying small molecules that could modulate its activity, thus offering insights into novel strategies for treating illnesses linked to impaired RNA processing. Overall, research on RPP40 not only deepens our understanding of fundamental molecular mechanisms but also highlights its potential as a target for future biomedical applications.
