Analytical Data
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Gene name
RNF186
- Application
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Alternative Names
RNF186; E3 ubiquitin-protein ligase RNF186; RING finger protein 186
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NXI6
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Expression Region
1-227 aa
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AA Sequence
MACTKTLQQSQPISAGATTTTTAVAPAGGHSGSTECDLECLVCREPYSCPRLPKLLACQHAFCAICLKLLLCVQDNTWSITCPLCRKVTAVPGGLICSLRDHEAVVGQLAQPCTEVSLCPQGLVDPADLAAGHPSLVGEDGQDEVSANHVAARRLAAHLLLLALLIILIGPFIYPGVLRWVLTFIIALALLMSTLFCCLPSTRGSCWPSSRTLFCREQKHSHISSIA
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Molecular Weight
50.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RNF186 is a relatively recent subject of investigation in the field of molecular biology, particularly due to its involvement in cellular processes such as protein degradation, cell signaling, and potential roles in diseases. As a member of the RING finger protein family, RNF186 is believed to function as an E3 ubiquitin ligase, which is crucial for the ubiquitination pathway that regulates protein turnover and cellular homeostasis. Research has suggested that RNF186 may play a significant role in various cancers and neurodegenerative diseases, making it a target of interest for therapeutic interventions. The elucidation of its structure and function, especially through the study of its recombinant protein, can provide insights into its mechanisms of action and the biological pathways it modulates. Additionally, the production of recombinant RNF186 allows for detailed biochemical analyses and assays to better understand its interactions with other cellular components, thus paving the way for potential drug development and novel treatment strategies. By enhancing our knowledge of RNF186, researchers aim to uncover its significance in health and disease, further illuminating the complex regulatory networks that underpin cellular function.
