Analytical Data
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基因名
RNF180
- Application
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别名
RNF180; E3 ubiquitin-protein ligase RNF180; EC 2.3.2.27; RING finger protein 180; RING-type E3 ubiquitin transferase RNF180
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q86T96
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表达区间
1-416 aa
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氨基酸序列
MKRSKELITKNHSQEETSILRCWKCRKCIASSGCFMEYLENQVIKDKDDSVDAQNICHVWHMNVEALPEWISCLIQKAQWTVGKLNCPFCGARLGGFNFVSTPKCSCGQLAAVHLSKSRTDYQPTQAGRLMRPSVKYLSHPRVQSGCDKEALLTGGGSENRNHRLLNMARNNNDPGRLTEALCLEVRPTYFEMKNEKLLSKASEPKYQLFVPQLVTGRCATRAFHRKSHSLDLNISEKLTLLPTLYEIHSKTTAYSRLNETQPIDLSGLPLQSSKNSYSFQNPSSFDPSMLLQRFSVAPHETQTQRGGEFQCGLEAASVYSDHTNTNNLTFLMDLPSAGRSMPEASDQEEHLSPLDFLHSANFSLGSINQRLNKRERSKLKNLRRKQRRRERWLQKQGKYSGVGLLDHMVSIYLLI
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分子量
73.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF180 is an E3 ubiquitin ligase that has garnered attention due to its potential implications in cancer biology and cellular signaling pathways. Research indicates that RNF180 may play a key role in regulating protein stability and degradation through the ubiquitin-proteasome system, which is essential for maintaining cellular homeostasis. Abnormal expression or mutations in RNF180 have been linked to various malignancies, prompting investigations into its function and mechanisms. Additionally, RNF180 is thought to interact with several proteins involved in critical cellular processes, such as apoptosis and cell cycle regulation, highlighting its significance in tumorigenesis. Given the increasing prevalence of cancer globally, understanding the molecular and functional dynamics of RNF180 could provide insights into novel therapeutic strategies. The study of recombinant RNF180 protein aims to unveil its structural characteristics, functional roles, and potential as a biomarker for cancer diagnosis and prognosis, ultimately contributing to the broader field of oncology and targeted treatment approaches.
