Analytical Data
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Gene name
phrB
- Application
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Alternative Names
phrB；(6-4) photolyase
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P00914
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Expression Region
1-472aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMGSMTTHLVWFRQDLRLHDNLALAAACRNS SARVLALYIATPRQWATHNMSPRQAELINAQLNGLQIALAEKGIPLLFRE VDDFVASVEIVKQVCAENSVTHLFYNYQYEVNERARDVEVERALRNVVCE GFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLKRLREGMPECVAAPKVRS SGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQNGAGEYEQQR DFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNELI WREFYRHLITYHPSLCKHRPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIV DAAMRQLNSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLA ANNGGWQWAASTGTDAAPYFRIFNPTTQGEKFDHEGEFIRQWLPELRDVP GKVVHEPWKWAQKAGVTLDYPQPIVEHKEARVQTLAAYEAARKGK
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Molecular Weight
56 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of the phrB recombinant protein is rooted in its significant role in bacterial communication and environmental adaptation. PhrB is a part of the quorum sensing system in certain bacteria, particularly in species like *Bacillus subtilis*, where it functions as a signaling molecule that regulates gene expression in response to population density. Understanding the structure and function of phrB can provide insights into the mechanisms of bacterial behavior, such as biofilm formation, virulence, and antibiotic resistance. The recombinant production of phrB enables researchers to investigate its biochemical properties and interactions with other cellular components in a controlled laboratory setting. Such studies can reveal how phrB contributes to bacterial survival and adaptability under various stress conditions, offering potential applications in biotechnology and medicine. For instance, modulating the activity of phrB or its pathways may lead to novel strategies for managing bacterial infections or enhancing the efficacy of probiotics. Overall, the investigation of phrB recombinant protein stands at the intersection of microbiology, genetics, and therapeutic development, highlighting its relevance in both fundamental and applied sciences.
