Analytical Data
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基因名
PDE1B
- Application
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别名
PDE1B；PDES1B；Dual specificity calcium/calmodulin-dependent 3'.5'-cyclic nucleotide phosphodiesterase 1B
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q01064
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表达区间
1-536aa
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氨基酸序列
MELSPRSPPEMLEESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEINIEELKKNLEYTASLLEAVYIDETRQILDTEDELQELRSDAVPSEVRDWLASTFTQQARAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTSVGPTYSTAVLNCLKNLDLWCFDVFSLNQAADDHALRTIVFELLTRHNLISRFKIPTVFLMSFLDALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIELLAIIFAAAIHDYEHTGTTNSFHIQTKSECAIVYNDRSVLENHHISSVFRLMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTALQQLERIDKPKALSLLLHAADISHPTKQWLVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLADEDSKSKNQPSFQWRQPSLDVEVGDPNPDVVSFRSTWVKRIQENKQKWKERAASGITNQMSIDELSPCEEEAPPSPAEDEHNQNGNLD
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分子量
86 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PDE1B (phosphodiesterase 1B) is an enzyme that belongs to the phosphodiesterase family, which plays a crucial role in cellular signaling by hydrolyzing cyclic nucleotides, such as cAMP and cGMP, thus regulating various physiological processes. Research into PDE1B has gained momentum due to its implications in neurodegenerative diseases, cognitive disorders, and cardiovascular conditions. Dysregulation of cyclic nucleotide levels, associated with PDE1B activity, has been linked to several pathologies, making this enzyme a potential therapeutic target. The study of recombinant PDE1B protein is particularly important for understanding its structure-function relationship and for the development of selective inhibitors that could offer new avenues for treatment. Additionally, the expression of recombinant PDE1B in various systems, such as bacteria or mammalian cells, facilitates detailed biochemical assays and structural studies, which are essential for drug discovery efforts. Insights gained through this research may lead to the identification of novel pharmacological agents, thereby contributing to the development of effective therapies for conditions exacerbated by altered cyclic nucleotide signaling. Overall, the investigation of PDE1B recombinant protein is a promising frontier in biomedical research, with the potential to yield significant advancements in the treatment of diseases influenced by this signaling pathway.
