Analytical Data
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Gene name
PDE1B
- Application
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Alternative Names
PDE1B；PDES1B；Dual specificity calcium/calmodulin-dependent 3'.5'-cyclic nucleotide phosphodiesterase 1B
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q01064
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Expression Region
1-536aa
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AA Sequence
MELSPRSPPEMLEESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEINIEELKKNLEYTASLLEAVYIDETRQILDTEDELQELRSDAVPSEVRDWLASTFTQQARAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTSVGPTYSTAVLNCLKNLDLWCFDVFSLNQAADDHALRTIVFELLTRHNLISRFKIPTVFLMSFLDALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIELLAIIFAAAIHDYEHTGTTNSFHIQTKSECAIVYNDRSVLENHHISSVFRLMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTALQQLERIDKPKALSLLLHAADISHPTKQWLVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLADEDSKSKNQPSFQWRQPSLDVEVGDPNPDVVSFRSTWVKRIQENKQKWKERAASGITNQMSIDELSPCEEEAPPSPAEDEHNQNGNLD
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Molecular Weight
86 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PDE1B (phosphodiesterase 1B) is an enzyme that belongs to the phosphodiesterase family, which plays a crucial role in cellular signaling by hydrolyzing cyclic nucleotides, such as cAMP and cGMP, thus regulating various physiological processes. Research into PDE1B has gained momentum due to its implications in neurodegenerative diseases, cognitive disorders, and cardiovascular conditions. Dysregulation of cyclic nucleotide levels, associated with PDE1B activity, has been linked to several pathologies, making this enzyme a potential therapeutic target. The study of recombinant PDE1B protein is particularly important for understanding its structure-function relationship and for the development of selective inhibitors that could offer new avenues for treatment. Additionally, the expression of recombinant PDE1B in various systems, such as bacteria or mammalian cells, facilitates detailed biochemical assays and structural studies, which are essential for drug discovery efforts. Insights gained through this research may lead to the identification of novel pharmacological agents, thereby contributing to the development of effective therapies for conditions exacerbated by altered cyclic nucleotide signaling. Overall, the investigation of PDE1B recombinant protein is a promising frontier in biomedical research, with the potential to yield significant advancements in the treatment of diseases influenced by this signaling pathway.
