Analytical Data
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基因名
RNF175
- Application
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别名
RNF175; RING finger protein 175
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8N4F7
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表达区间
1-328 aa
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氨基酸序列
MAAGTAARKAAPVLEAPPQQEQLSHTKLSAEDTWNLQQERMYKMHRGHDSMHVEMILIFLCVLVIAQIVLVQWRQRHGRSYNLVTLLQMWVVPLYFTIKLYWWRFLSMWGMFSVITSYILFRATRKPLSGRTPRLVYKWFLLIYKLSYAFGVVGYLAIMFTMCGFNLFFKIKARDSMDFGIVSLFYGLYYGVMGRDFAEICSDYMASTIGFYSVSRLPTRSLSDNICAVCGQKIIVELDEEGLIENTYQLSCNHVFHEFCIRGWCIVGKKQTCPYCKEKVDLKRMISNPWERTHFLYGQILDWLRYLVAWQPVVIGIVQGINYSLGLE
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分子量
64.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF175, also known as RING Finger Protein 175, is an E3 ubiquitin ligase that has garnered attention in recent years due to its potential roles in various cellular processes, including protein degradation, cell cycle regulation, and cellular response to stress. The study of RNF175 is critical as it is implicated in several diseases, including cancer, where dysregulation of ubiquitination pathways can lead to tumorigenesis. Research has shown that RNF175 interacts with various substrates, affecting their stability and localization within the cell. Its unique RING domain facilitates the transfer of ubiquitin moieties from E2 conjugating enzymes to target proteins, thereby marking them for proteasomal degradation or influencing their functional activity. Moreover, alterations in the expression levels or mutations in RNF175 have been associated with pathological conditions, emphasizing its potential as a therapeutic target. By producing recombinant RNF175 protein, scientists aim to elucidate its biological functions, understand its mechanisms of action, and explore its potential as a biomarker or therapeutic intervention in diseases characterized by aberrant protein homeostasis. Overall, the study of RNF175 not only enhances our understanding of the ubiquitin-proteasome system but also provides insights into the complex regulatory networks that govern cellular functions and their contributions to human health and disease.
