Analytical Data
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Gene name
GAD2
- Application
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Alternative Names
GAD2；GAD65；Glutamate decarboxylase 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q05329
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Expression Region
1-585aa
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AA Sequence
MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYG DAEKPAESGGSQPPRAAARKAACAC DQKPCSCSKVDVNYAFLHATDLL PACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWE LA DQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWL TSTANTNMFTYEIAPVFVLLEYVTLKKMR EIIGWPGGSGDGIFSPGGA ISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGI GTDSVI LIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYG AFDPLLAVADICKKYKIWMHVDAAWGGGLLMSR KHKWKLSGVERANSV TWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTG DKALQCGRHV DVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNR EGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSR LSKVAPVIKAR MMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL
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Molecular Weight
90 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GAD2 (Glutamate Decarboxylase 2) is a key enzyme responsible for the synthesis of gamma-aminobutyric acid (GABA), a principal inhibitory neurotransmitter in the central nervous system. GAD2 predominantly exists in pancreatic beta cells, where it plays a crucial role in regulating insulin secretion and maintaining glucose homeostasis. Dysregulation of GAD2 has been implicated in various neurological disorders, including anxiety, epilepsy, and schizophrenia, as well as autoimmune diabetes, where antibodies against GAD2 can lead to beta-cell destruction. Given its significant role in both neurobiology and endocrinology, recombinant GAD2 protein has garnered attention for its potential applications in research and therapy. The production of this protein through recombinant DNA technology allows for a detailed investigation into its enzymatic properties, regulation, and interaction with other neuroactive substances. Additionally, understanding the functional mechanisms of GAD2 opens new avenues for developing therapeutic strategies aimed at mitigating the effects of disorders associated with GABAergic dysfunction. Researchers are increasingly exploring the therapeutic potential of GAD2 in autoimmune diabetes, harnessing its role as an autoantigen in immunotherapy approaches. Overall, the study of recombinant GAD2 protein is pivotal for elucidating its multifaceted roles in health and disease, fostering advancements in both basic research and clinical applications.
