Analytical Data
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Gene name
malE
- Application
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Alternative Names
malE；MSL1L1；Male-specific lethal 1 homolog
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0AEX9
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Expression Region
27-396aa
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AA Sequence
KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITK
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Molecular Weight
48.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of malE recombinant proteins primarily revolves around the malE gene, which encodes the maltose-binding protein (MBP) in *Escherichia coli*. MBP plays a crucial role in the transport of maltose and other carbohydrates across the bacterial membrane, serving as a prokaryotic periplasmic binding protein. In recombinant protein technology, the malE gene is often used as a tag for the purification of target proteins, facilitating their isolation through affinity chromatography. Researchers exploit this property to produce MBP-tagged proteins, thereby enhancing yield and solubility. This approach has significant implications in biotechnology and pharmaceutical industries, as it enables the production of proteins with therapeutic potential, structural studies, and functional assays. Moreover, the malE system can be beneficial in studying protein-protein interactions, enzymatic activity, and structural biology. The versatility of malE in various expression systems and its ability to improve protein stability emphasize its importance in molecular biology research. By continuing to explore malE recombinant proteins, scientists aim to advance their understanding of protein dynamics and enhance the development of novel therapeutic agents.
