Analytical Data
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基因名
RNF146
- Application
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别名
Dactylidin; dJ351K20.1 ; DKFZP434O1427; E3 ubiquitin-protein ligase rnf146; RING finger protein 146; RN146_HUMAN; RNF 146; Rnf146; RP3 351K20.1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NTX7
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表达区间
1-359 aa
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氨基酸序列
MMAGCGEIDH SINMLPTNRK ANESCSNTAP SLTVPECAIC LQTCVHPVSL PCKHVFCYLC VKGASWLGKR CALCRQEIPE DFLDKPTLLS PEELKAASRG NGEYAWYYEG RNGWWQYDER TSRELEDAFS KGKKNTEMLI AGFLYVADLE NMVQYRRNEH GRRRKIKRDI IDIPKKGVAG LRLDCDANTV NLARESSADG ADSVSAQSGA SVQPLVSSVR PLTSVDGQLT SPATPSPDAS TSLEDSFAHL QLSGDNTAER SHRGEGEEDH ESPSSGRVPA PDTSIEETES DASSDSEDVS AVVAQHSLTQ QRLLVSNANQ TVPDRSDRSG TDRSVAGGGT VSVSVRSRRP DGQCTVTEV
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分子量
38.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF146, also known as Ring Finger Protein 146, is an E3 ubiquitin ligase that plays a pivotal role in various cellular processes, including the regulation of protein degradation, cell cycle progression, and signal transduction. Its involvement in ubiquitination pathways has garnered significant attention, particularly in the context of cancer and neurodegenerative diseases, where dysregulation of protein homeostasis is a common feature. RNF146 is known to mediate the ubiquitination of several key substrates, thereby affecting their stability and function. Recent studies have suggested that RNF146 may be implicated in the modulation of cellular stress responses, particularly under conditions of oxidative stress, further emphasizing its potential as a therapeutic target. The generation of recombinant RNF146 protein facilitates detailed investigations into its biochemical properties, interactions with substrates, and functional implications in various signaling pathways. Understanding the molecular mechanisms of RNF146 could provide insights into its role in disease pathology and may pave the way for the development of novel strategies for intervention in diseases characterized by protein misfolding and aggregation. As such, research on RNF146 is poised to contribute significantly to the fields of molecular biology and therapeutic development.
