Analytical Data
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基因名
Smad5
- Application
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别名
Smad5；MADH5；Mothers against decapentaplegic homolog 5
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种属
Mouse
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P97454
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表达区间
2-465aa
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氨基酸序列
TSMASLFSF TSPAVKRLLG WKQGDEEEKW AEKAVDALVK KLKKKKGAME ELEKALSSPG QPSKCVTIPR SLDGRLQVSH RKGLPHVIYC RVWRWPDLQS HHELKPLDIC EFPFGSKQKE VCINPYHYKR VESPVLPPVL VPRHNEFNPQ HSLLVQFRNL SHNEPHMPQN ATFPDSFHQP NNAPFPLSPN SPYPPSPASS TYPNSPASSG PGSPFQLPAD TPPPAYMPPD DQMAPDNSQP MDTSSNMIPQ TMPSISSRDV QPVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT DPSNNKSRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD SSIFVQSRNC NFHHGFHPTT VCKIPSSCSL KIFNNQEFAQ LLAQSVNHGF EAVYELTKMC TIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV LTQMGSPLNP ISSVS
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Smad5 is a critical protein in the TGF-beta (transforming growth factor-beta) signaling pathway, which plays a vital role in regulating various biological processes, including cell growth, differentiation, and apoptosis. Its importance is underscored by its involvement in embryonic development and the immune response. Research into the recombinant form of Smad5 has gained traction as scientists aim to elucidate its specific functions and interactions within the broader signaling network. Recombinant proteins, such as Smad5, provide researchers with the ability to study their biochemical properties in controlled settings, enabling detailed analyses of their structural and functional roles. Furthermore, understanding Smad5's mechanisms can contribute to insights into various pathologies, including cancer and fibrotic diseases, where TGF-beta signaling is often dysregulated. By generating recombinant Smad5, researchers can investigate its interactions with other signaling molecules, study the effects of post-translational modifications, and explore its potential as a therapeutic target. This line of study not only helps in unveiling the intricate regulatory mechanisms of cellular signaling but also paves the way for developing novel treatment strategies aimed at modulating TGF-beta-related pathways in disease contexts.
