Analytical Data
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Gene name
cry3Aa
- Application
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Alternative Names
cry3Aa；bt13；cry3A；cryC；Pesticidal crystal Protein Cry3Aa
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A379
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Expression Region
58-644aa
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AA Sequence
DSSTTKDVIQKGISVVGDLLGVVGFPFGGALVSFYTNFLNTIWPSEDPWKAFMEQVEALMDQKIADYAKNKALAELQGLQNNVEDYVSALSSWQKNPVSSRNPHSQGRIRELFSQAESHFRNSMPSFAISGYEVLFLTTYAQAANTHLFLLKDAQIYGEEWGYEKEDIAEFYKRQLKLTQEYTDHCVKWYNVGLDKLRGSSYESWVNFNRYRREMTLTVLDLIALFPLYDVRLYPKEVKTELTRDVLTDPIVGVNNLRGYGTTFSNIENYIRKPHLFDYLHRIQFHTRFQPGYYGNDSFNYWSGNYVSTRPSIGSNDIITSPFYGNKSSEPVQNLEFNGEKVYRAVANTNLAVWPSAVYSGVTKVEFSQYNDQTDEASTQTYDSKRNVGAVSWDSIDQLPPETTDEPLEKGYSHQLNYVMCFLMQGSRGTIPVLTWTHKSVDFFNMIDSKKITQLPLVKAYKLQSGASVVAGPRFTGGDIIQCTENGSAATIYVTPDVSYSQKYRARIHYASTSQITFTLSLDGAPFNQYYFDKTINKGDTLTYNSFNLASFSTPFELSGNNLQIGVTGLSAGDKVYIDKIEFIPVN
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Molecular Weight
84.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Cry3Aa is a member of the Cry protein family produced by the bacterium Bacillus thuringiensis (Bt), which has garnered significant attention due to its insecticidal properties against certain pests, particularly within the Coleoptera order, such as the Colorado potato beetle. The increasing demand for sustainable agricultural practices has highlighted the need for effective biopesticides that can mitigate the reliance on chemical pesticides, which often lead to environmental and health concerns. Research into the structural and functional aspects of Cry3Aa has revealed insights into its mechanism of action and potential for enhancing pest resistance in crops. The recombinant expression of Cry3Aa in various systems allows for detailed studies on its efficacy, stability, and safety. Additionally, efforts are underway to improve its potency and broaden its spectrum of activity through genetic engineering techniques, enabling the development of transgenic plants that incorporate Cry3Aa to achieve improved pest resistance. This research not only provides a sustainable solution for crop protection but also contributes to the understanding of insect-host interactions, laying the groundwork for future advancements in agricultural biotechnology. The exploration of Cry3Aa and its derivatives continues to be pivotal in addressing global food security challenges while promoting environmentally friendly farming practices.
