Analytical Data
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Gene name
RKHD3
- Application
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Alternative Names
MEX3B; KIAA2009; RKHD3; RNF195RNA-binding protein MEX3B; RING finger and KH domain-containing protein 3; RING finger protein 195
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6ZN04
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Expression Region
1-569 aa
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AA Sequence
MPSSLFADLE RNGSGGGGGG SSGGGETLDD QRALQLALDQ LSLLGLDSDE GASLYDSEPR KKSVNMTECV PVPSSEHVAE IVGRQGCKIK ALRAKTNTYI KTPVRGEEPV FVVTGRKEDV AMARREIISA AEHFSMIRAS RNKNTALNGA VPGPPNLPGQ TTIQVRVPYR VVGLVVGPKG ATIKRIQQQT HTYIVTPSRD KEPVFEVTGM PENVDRAREE IEAHIALRTG GIIELTDEND FHANGTDVGF DLHHGSGGSG PGSLWSKPTP SITPTPGRKP FSSYRNDSSS SLGSASTDSY FGGGTSSSAA ATQRLADYSP PSPALSFAHN GNNNNNGNGY TYTAGGEASV PSPDGCPELQ PTFDPAPAPP PGAPLIWAQF ERSPGGGPAA PVSSSCSSSA SSSASSSSVV FPGGGASAPS NANLGLLVHR RLHPGTSCPR LSPPLHMAPG AGEHHLARRV RSDPGGGGLA YAAYANGLGA QLPGLQPSDT SGSSSSSSSS SSSSSSSSGL RRKGSRDCSV CFESEVIAAL VPCGHNLFCM ECANRICEKS EPECPVCHTA VTQAIRIFS
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Molecular Weight
58.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RKHD3, a member of the Rho GTPase-activating protein family, has garnered attention in the field of molecular biology due to its potential roles in cellular signaling, proliferation, and differentiation. Emerging evidence suggests that RKHD3 may play a significant role in regulating acts' dynamics of the cytoskeleton and influencing cell migration and adhesion, critical processes in various physiological and pathological conditions, including cancer metastasis and wound healing. Recent studies have indicated a correlation between RKHD3 expression levels and tumor progression, suggesting that it may serve as a potential biomarker for cancer diagnostics or therapy. Additionally, research into RKHD3's mechanism of action has unveiled its involvement in the modulation of Rho family GTPases, thereby affecting downstream signaling pathways crucial for cell behavior. As such, the recombinant expression and purification of RKHD3 protein is essential for further elucidating its functional roles, interactions, and potential therapeutic targets. Understanding the biochemical characteristics and regulatory mechanisms of RKHD3 can not only enhance our knowledge of its biological significance but also pave the way for novel strategies in cancer treatment and regenerative medicine. Thus, investigations into RKHD3 are pivotal for both basic research and translational applications in the context of cell biology and oncology.
