Analytical Data
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基因名
CARD4
- Application
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别名
CARD 4; CARD4; Caspase recruitment domain 4; Caspase recruitment domain containing Protein 4; Caspase recruitment domain family member 4; Caspase recruitment domain Protein 4; Caspase recruitment domain-containing Protein 4; CLR 7.1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y239
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表达区间
1-953aa
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氨基酸序列
MEEQGHSEMEIIPSESHPHIQLLKSNRELLVTHIRNTQCLVDNLLKNDYFSAEDAEIVCACPTQPDKVRKILDLVQSKGEEVSEFFLYLLQQLADAYVDLRPWLLEIGFSPSLLTQSKVVVNTDPVSRYTQQLRHHLGRDSKFVLCYAQKEELLLEEIYMDTIMELVGFSNESLGSLNSLACLLDHTTGILNEQGETIFILGDAGVGKSMLLQRLQSLWATGRLDAGVKFFFHFRCRMFSCFKESDRLCLQDLLFKHYCYPERDPEEVFAFLLRFPHVALFTFDGLDELHSDLDLSRVPDSSCPWEPAHPLVLLANLLSGKLLKGASKLLTARTGIEVPRQFLRKKVLLRGFSPSHLRAYARRMFPERALQDRLLSQLEANPNLCSLCSVPLFCWIIFRCFQHFRAAFEGSPQLPDCTMTLTDVFLLVTEVHLNRMQPSSLVQRNTRSPVETLHAGRDTLCSLGQVAHRGMEKSLFVFTQEEVQASGLQERDMQLGFLRALPELGPGGDQQSYEFFHLTLQAFFTAFFLVLDDRVGTQELLRFFQEWMPPAGAATTSCYPPFLPFQCLQGSGPAREDLFKNKDHFQFTNLFLCGLLSKAKQKLLRHLVPAAALRRKRKALWAHLFSSLRGYLKSLPRVQVESFNQVQAMPTFIWMLRCIYETQSQKVGQLAARGICANYLKLTYCNACSADCSALSFVLHHFPKRLALDLDNNNLNDYGVRELQPCFSRLTVLRLSVNQITDGGVKVLSEELTKYKIVTYLGLYNNQITDVGARYVTKILDECKGLTHLKLGKNKITSEGGKYLALAVKNSKSISEVGMWGNQVGDEGAKAFAEALRNHPSLTTLSLASNGISTEGGKSLARALQQNTSLEILWLTQNELNDEVAESLAEMLKVNQTLKHLWLIQNQITAKGTAQLADALQSNTGITEICLNGNLIKPEEAKVYEDEKRIICF
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分子量
107.6 KDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CARD4, also known as the caspase activation and recruitment domain-containing protein 4, plays a pivotal role in the innate immune response by sensing intracellular pathogens and mediating inflammatory responses. As a member of the NOD-like receptor (NLR) family, CARD4 is primarily localized in the cytoplasm and is involved in the detection of bacterial components, leading to the activation of downstream signaling pathways, including the NF-κB and MAPK pathways. Research into CARD4 has gained momentum due to its association with various inflammatory and autoimmune diseases, such as Crohn’s disease and systemic lupus erythematosus. The ability of CARD4 to form inflammasomes, which are multiprotein complexes that activate caspase-1 and promote the processing of pro-inflammatory cytokines, highlights its importance in regulating inflammation and cell death. Advances in structural biology and protein engineering have facilitated the understanding of CARD4’s functional mechanisms, thus opening avenues for potential therapeutic interventions aimed at modulating its activity. Moreover, studies on CARD4’s protein interactions and regulatory mechanisms are crucial for deciphering its role in host defense and the pathogenesis of related diseases. Given the dual nature of CARD4 in promoting inflammation while also serving protective roles against pathogen invasion, it continues to be a significant focus of research in immunology and cell biology.
