Analytical Data
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Gene name
amiB2
- Application
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Alternative Names
amiB2；Putative amidase AmiB2
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P9WQ97
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Expression Region
1-462aa
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AA Sequence
MDPTDLAFAGAAAQARMLADGALTAPMLLEVYLQRIERLDSHLRAYRVVQFDRARAEAEAAQQRLDAGERLPLLGVPIAIKDDVDIAGEVTTYGSAGHGPAATSDAEVVRRLRAAGAVIIGKTNVPELMIMPFTESLAFGATRNPWCLNRTPGGSSGGSAAAVAAGLAPVALGSDGGGSIRIPCTWCGLFGLKPQRDRISLEPHDGAWQGLSVNGPIARSVMDAALLLDATTTVPGPEGEFVAAAARQPGRLRIALSTRVPTPLPVRCGKQELAAVHQAGALLRDLGHDVVVRDPDYPASTYANYLPRFFRGISDDADAQAHPDRLEARTRAIARLGSFFSDRRMAALRAAEVVLSSRIQSIFDDVDVVVTPGAATGPSRIGAYQRRGAVSTLLLVVQRVPYFQVWNLTGQPAAVVPWDFDGDGLPMSVQLVGRPYDEATLLALAAQIESARPWAHRRPSVS
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Molecular Weight
69.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AmiB2 is a recombinant protein derived from the bacterium *Bacillus amyloliquefaciens*, known for its ability to degrade amylose, a major component of starch. The research into AmiB2 has gained traction due to the increasing industrial demand for enzymes that can efficiently convert starch into simpler sugars for various applications, including biofuel production, food processing, and biodegradable plastics. With the growing need for sustainable solutions in these sectors, AmiB2 presents a promising candidate for biocatalysis due to its unique enzymatic properties, stability, and versatility. Its potential applications are particularly relevant in the context of developing environmentally friendly methods for starch hydrolysis, which aligns with global trends toward sustainability and green chemistry. Furthermore, as researchers delve into the molecular structure and functional mechanisms of AmiB2, they aim to enhance its performance through genetic engineering and protein engineering techniques. This could lead to the creation of more efficient variants of the enzyme, thereby optimizing production processes across various industries. Overall, the study of AmiB2 not only addresses practical industrial challenges but also contributes to the broader field of enzyme research, paving the way for innovative applications in biotechnology and environmental science.
