Analytical Data
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基因名
RFWD3
- Application
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别名
BC027246; E3 ubiquitin protein ligase RFWD3; E3 ubiquitin-protein ligase RFWD3; FLJ10520; MGC27888; RFWD3; RFWD3_HUMAN; Ring finger and WD repeat domain 3; RING finger and WD repeat domain containing protein 3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201; RNF201
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q6PCD5
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表达区间
1-774 aa
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氨基酸序列
MAHEAMEYDV QVQLNHAEQQ PAPAGMASSQ GGPALLQPVP ADVVSSQGVP SILQPAPAEV ISSQATPPLL QPAPQLSVDL TEVEVLGEDT VENINPRTSE QHRQGSDGNH TIPASSLHSM TNFISGLQRL HGMLEFLRPS SSNHSVGPMR TRRRVSASRR ARAGGSQRTD SARLRAPLDA YFQVSRTQPD LPATTYDSET RNPVSEELQV SSSSDSDSDS SAEYGGVVDQ AEESGAVILE EQLAGVSAEQ EVTCIDGGKT LPKQPSPQKS EPLLPSASMD EEEGDTCTIC LEQWTNAGDH RLSALRCGHL FGYRCISTWL KGQVRKCPQC NKKARHSDIV VLYARTLRAL DTSEQERMKS SLLKEQMLRK QAELESAQCR LQLQVLTDKC TRLQRRVQDL QKLTSHQSQN LQQPRGSQAW VLSCSPSSQG QHKHKYHFQK TFTVSQAGNC RIMAYCDALS CLVISQPSPQ ASFLPGFGVK MLSTANMKSS QYIPMHGKQI RGLAFSSYLR GLLLSASLDN TIKLTSLETN TVVQTYNAGR PVWSCCWCLD EANYIYAGLA NGSILVYDVR NTSSHVQELV AQKARCPLVS LSYMPRAASA AFPYGGVLAG TLEDASFWEQ KMDFSHWPHV LPLEPGGCID FQTENSSRHC LVTYRPDKNH TTIRSVLMEM SYRLDDTGNP ICSCQPVHTF FGGPTCKLLT KNAIFQSPEN DGNILVCTGD EAANSALLWD AASGSLLQDL QTDQPVLDIC PFEVNRNSYL ATLTEKMVHI YKWE
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分子量
85.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The research on RFWD3, a member of the RING finger E3 ubiquitin ligase family, has gained significant attention due to its crucial roles in cellular processes such as DNA damage response, repair, and cell cycle regulation. Emerging evidence suggests that RFWD3 plays a pivotal role in maintaining genomic stability by facilitating the ubiquitination of various substrates involved in these vital processes. Dysfunction or mutations in RFWD3 have been linked to increased susceptibility to cancers and other diseases, underscoring its importance in tumor suppression. Additionally, recent studies highlight RFWD3's interactions with key proteins like RAD51, which is essential for homologous recombination repair. Given the growing awareness of the intricate relationship between the ubiquitin-proteasome system and cellular stress responses, understanding RFWD3's signaling pathways could reveal novel therapeutic targets for cancer treatment. The exploration of RFWD3's structure and function may also provide insights into the mechanisms by which cells respond to genotoxic events and how aberrations in these pathways contribute to the onset of malignancies. Thus, research on RFWD3 not only advances our comprehension of fundamental biological processes but also holds promise for developing innovative strategies in cancer therapy.
