Analytical Data
-
Gene name
ABHD4
- Application
-
Alternative Names
ABHD4；(Lyso)-N-acylphosphatidylethanolamine lipase
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q8TB40
-
Expression Region
1-342
-
AA Sequence
MADDLEQQSQ GWLSSWLPTW RPTSMSQLKN VEARILQCLQ NKFLARYVSL PNQNKIWTVT VSPEQNDRTP LVMVHGFGGG VGLWILNMDS LSARRTLHTF DLLGFGRSSR PAFPRDPEGA EDEFVTSIET WRETMGIPSM ILLGHSLGGF LATSYSIKYP DRVKHLILVD PWGFPLRPTN PSEIRAPPAW VKAVASVLGR SNPLAVLRVA GPWGPGLVQR FRPDFKRKFA DFFEDDTISE YIYHCNAQNP SGETAFKAMM ESFGWARRPM LERIHLIRKD VPITMIYGSD TWIDTSTGKK VKMQRPDSYV RDMEIKGASH HVYADQPHIF NAVVEEICDS VD
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ABHD4 (Alpha/Beta Hydrolase Domain Containing 4) is a member of the alpha/beta hydrolase fold superfamily, which plays a crucial role in lipid metabolism and cellular signaling pathways. The protein is known for its involvement in the hydrolysis of various lipid substrates, making it a significant target of research in the context of metabolic disorders and inflammation. Recent studies have implicated ABHD4 in the regulation of endocannabinoid signaling, particularly in the degradation of 2-arachidonoylglycerol (2-AG), an important endocannabinoid that modulates synaptic transmission and affects pain perception, appetite, and mood. Understanding the structure and function of ABHD4 through recombinant protein studies can provide insights into its enzymatic mechanisms and substrate specificity, potentially leading to the discovery of novel therapeutic strategies for diseases related to dysregulated lipid metabolism, such as obesity and neuropathic pain. Furthermore, the development of specific inhibitors or modulators of ABHD4 activity may offer new avenues for manipulating cannabinoid signaling in a clinical setting. Comprehensive investigations of ABHD4 recombinant protein are therefore essential for elucidating its biological roles and therapeutic potentials.
