Analytical Data
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Gene name
RFP2
- Application
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Alternative Names
TRIM13; LEU5; RFP2; RNF77; E3 ubiquitin-protein ligase TRIM13; B-cell chronic lymphocytic leukemia tumor suppressor Leu5; Leukemia-associated protein 5; Putative tumor suppressor RFP2; RING finger protein 77; RING-type E3 ubiquitin transferase TRIM13; Ret finger protein 2; Tripartite motif-containing protein 13
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60858
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Expression Region
1-410 aa
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AA Sequence
MDVMELLEEDLTCPICCSLFDDPRVLPCSHNFCKKCLEGILEGSVRNSLWRPAPFKCPTCRKETSATGINSLQVNYSLKGIVEKYNKIKISPKMPVCKGHLGQPLNIFCLTDMQLICGICATRGEHTKHVFCSIEDAYAQERDAFESLFQSFETWRRGDALSRLDTLETSKRKSLQLLTKDSDKVKEFFEKLQHTLDQKKNEILSDFETMKLAVMQAYDPEINKLNTILQEQRMAFNIAEAFKDVSEPIVFLQQMQEFREKIKVIKETPLPPSNLPASPLMKNFDTSQWEDIKLVDVDKLSLPQDTGTFISKIPWSFYKLFLLILLLGLVIVFGPTMFLEWSLFDDLATWKGCLSNFSSYLTKTADFIEQSVFYWEQVTDGFFIFNERFKNFTLVVLNNVAEFVCKYKLL
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Molecular Weight
73.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RFP2, a member of the ring finger protein family, plays a crucial role in various cellular processes, including DNA repair, cell cycle regulation, and protein degradation. Its unique structural features, characterized by a RING-finger domain, allow it to function as an E3 ubiquitin ligase, facilitating the transfer of ubiquitin molecules to target proteins. This post-translational modification is essential for proteasomal degradation and the modulation of protein activity, ultimately influencing numerous signaling pathways. Research on RFP2 has gained momentum due to its implications in cancer biology, where aberrant expression or mutations can lead to disrupted cellular homeostasis and tumorigenesis. Furthermore, the interaction of RFP2 with other cellular partners suggests its involvement in complex regulatory networks. Understanding the molecular mechanisms underlying RFP2 functionality not only sheds light on its physiological roles but also opens avenues for therapeutic interventions, particularly in cancer. The exploration of RFP2 as a potential biomarker or target for novel drugs is crucial in the development of strategies to combat malignancies linked to its dysregulation. As such, the study of RFP2 and its recombinant protein form is pivotal in elucidating the intricate balance of cellular processes and addressing challenges in targeted cancer therapies.
