Analytical Data
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基因名
MAP2K1
- Application
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别名
MAP2K1；MEK1；PRKMK1；Dual specificity mitogen-activated Protein kinase kinase 1
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种属
Mouse
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P31938
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表达区间
2-393aa
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氨基酸序列
PKKKPTPIQ LNPAPDGSAV NGTSSAETNL EALQKKLEEL ELDEQQRKRL EAFLTQKQKV GELKDDDFEK ISELGAGNGG VVFKVSHKPS GLVMARKLIH LEIKPAIRNQ IIRELQVLHE CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS LDQVLKKAGR IPEQILGKVS IAVIKGLTYL REKHKIMHRD VKPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY SVQSDIWSMG LSLVEMAVGR YPIPPPDAKE LELLFGCHVE GDAAETPPRP RTPGRPLSSY GMDSRPPMAI FELLDYIVNE PPPKLPSGVF SLEFQDFVNK CLIKNPAERA DLKQLMVHAF IKRSDAEEVD FAGWLCSTIG LNQPSTPTHA ASI
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MAP2K1, also known as MEK1, is a critical component of the MAPK/ERK signaling pathway, which plays a vital role in regulating various cellular processes, including proliferation, differentiation, and survival. Abnormalities in this pathway, particularly mutations in MAP2K1, have been implicated in various human diseases, including cancers and developmental disorders. The study of MAP2K1 has garnered significant attention due to its role in the pathogenesis of tumors, such as melanoma and colorectal cancer, where it is often found mutated or overexpressed. Furthermore, MAP2K1 serves as a crucial target for therapeutic interventions, as inhibiting its activity can disrupt aberrant signaling pathways driving tumor growth. Researchers have been focusing on the structural characterization and functional analysis of recombinant MAP2K1 proteins to better understand their biochemical properties and interactions. This research not only aids in deciphering the molecular mechanisms underlying MAP2K1-related diseases but also facilitates the development of targeted therapies aimed at selectively modulating its activity. Understanding the intricate dynamics of MAP2K1 may ultimately provide insights into novel therapeutic strategies for diseases associated with its dysregulation, paving the way for more effective treatments in oncology and beyond.
