Analytical Data
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Gene name
RBM15
- Application
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Alternative Names
One twenty two protein ; One-twenty two protein 1; OTT; OTT1; Putative RNA-binding protein 15; RBM15; RBM15_HUMAN; RNA binding motif protein 15; RNA-binding motif protein 15
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96T37
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Expression Region
1-563 aa
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AA Sequence
MPEDDQRATRNLFIGNLDHSVSEVELRRAFEKYGIIEEVVIKRPARGQGGAYAFLKFQNLDMAHRAKVAMSGRVIGRNPIKIGYGKANPTTRLWVGGLGPNTSLAALAREFDRFGSIRTIDHVKGDSFAYIQYESLDAAQAACAKMRGFPLGGPDRRLRVDFAKAEETRYPQQYQPSPLPVHYELLTDGYTRHRNLDADLVRDRTPPHLLYSDRDRTFLEGDWTSPSKSSDRRNSLEGYSRSVRSRSGERWGADGDRGLPKPWEERRKRRSLSSDRGRTTHSPYEERSRTKGSGQQSERGSDRTPERSRKENHSSEGTKESSSNSLSNSRHGAEERGHHHHHHEAADSSHGKKARDSERNHRTTEAEPKPLEEPKHETKKLKNLSEYAQTLQLGWNGLLVLKNSCFPTSMHILEGDQGVISSLLKDHTSGSKLTQLKIAQRLRLDQPKLDEVTRRIKQGSPNGYAVLLATQATPSGLGTEGMPTVEPGLQRRLLRNLVSYLKQKQAAGVISLPVGGSKGRDGTGMLYAFPPCDFSQQYLQSALRTLGKLEEEHMVIVIVRDTA
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Molecular Weight
88.33 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RBM15 (RNA-binding motif protein 15) is a key regulator in various biological processes, including RNA splicing, gene expression, and cell differentiation. Research into RBM15 has gained momentum due to its significant role in hematopoiesis and its association with specific hematological malignancies. The protein is part of the splicing factor complex and is implicated in the regulation of alternative splicing events, which can impact cell fate decisions. Recent studies have highlighted the importance of RBM15 in maintaining stem cell properties and its involvement in the development of acute myeloid leukemia (AML), where it may act as an oncogenic factor. The understanding of RBM15 function is further complicated by the presence of several isoforms and the interplay with other regulatory proteins. Furthermore, the study of RBM15's role in gene expression regulation and its potential as a therapeutic target underscores the need for detailed analysis of its domain structure and interaction networks. Consequently, the generation of recombinant RBM15 protein has become a focal point of research, enabling researchers to dissect its molecular functions and explore its potential roles in disease mechanisms and novel therapeutic strategies. By investigating the biochemical properties and interactions of RBM15, researchers aim to elucidate its contributions to normal cellular processes and how its dysregulation may lead to pathologies, thereby opening new avenues for targeted therapies in cancers and other diseases where RBM15 plays a crucial role.
