Analytical Data
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Gene name
C9orf114
- Application
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Alternative Names
SPOUT1; C9orf114Putative methyltransferase C9orf114; EC 2.1.1.-; Centromere Protein 32; CENP-32; Kinetochore-associated Protein; SPOUT domain-containing methyltransferase 1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5T280
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Expression Region
1-376aa
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AA Sequence
MAERGRKRPCGPGEHGQRIEWRKWKQQKKEEKKKWKDLKLMKKLERQRAQEEQAKRLEEEEAAAEKEDRGRPYTLSVALPGSILDNAQSPELRTYLAGQIARACAIFCVDEIVVFDEEGQDAKTVEGEFRGVGKKGQACVQLARILQYLECPQYLRKAFFPKHQDLQFAGLLNPLDSPHHMRQDEESEFREGIVVDRPTRPGHGSFVNCGMKKEVKIDKNLEPGLRVTVRLNQQQHPDCKTYHGKVVSSQDPRTKAGLYWGYTVRLASCLSAVFAEAPFQDGYDLTIGTSERGSDVASAQLPNFRHALVVFGGLQGLEAGADADPNLEVAEPSVLFDLYVNTYPGQGSRTIRTEEAILISLAALQPGLIQAGARHT
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Molecular Weight
68.5 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
C9orf114, also known as Chromosome 9 Open Reading Frame 114, is a gene that has garnered attention in the field of molecular biology and genetics due to its potential implications in various diseases. Recent studies have suggested that the protein encoded by C9orf114 may play a role in cellular processes such as gene expression regulation, cell differentiation, and immune responses. Its expression has been observed in various tissues, indicating a potential multifunctional role in vivo. Moreover, emerging evidence points towards its involvement in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), particularly in relation to the infamous hexanucleotide repeat expansion in C9orf72, which is located nearby. The creation and study of recombinant C9orf114 protein are crucial for understanding its structure and function, as well as its interaction with other proteins and cellular pathways. By utilizing recombinant DNA technology, researchers can produce this protein in a controlled environment, allowing for detailed biochemical assays and functional analyses. Understanding the biological function of C9orf114 and its potential pathophysiological roles could pave the way for novel therapeutic targets and strategies for disease intervention, thereby contributing significantly to the fields of neurobiology and genetics. As such, C9orf114 represents a promising area of investigation for both basic research and clinical applications.
