Analytical Data
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Gene name
RBL1
- Application
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Alternative Names
107 kDa retinoblastoma-associated protein; Cellular protein 107; Cellular protein p107; CP107; MGC40006; p107; PRB1; RBL1; RBL1_HUMAN; Retinoblastoma like protein 1; retinoblastoma-like 1 (p107); Retinoblastoma-like protein 1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P28749
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Expression Region
1-429 aa
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AA Sequence
MTAALAVVTTSGLEDGVPRSRGEGTGEVVLERGPGAAYHMFVVMEDLVEKLKLLRYEEEFLRKSNLKAPSRHYFALPTNPGEQFYMFCTLAAWLINKAGRPFEQPQEYDDPNATISNILSELRSFGRTADFPPSKLKSGYGEHVCYVLDCFAEEALKYIGFTWKRPIYPVEELEEESVAEDDAELTLNKVDEEFVEEETDNEENFIDLNVLKAQTYHLDMNETAKQEDILESTTDAAEWSLEVERVLPQLKVTIRTDNKDWRIHVDQMHQHRSGIESALKETKGFLDKLHNEITRTLEKISSREKYINNQLENLVQEYRAAQAQLSEAKERYQQGNGGVTERTRLLSEVMEELEKVKQEMEEKGSSMTDGAPLVKIKQSLTKLKQETVEMDIRIGIVEHTLLQSKLKEKSNMTRNMHATVIPEPATGFY
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Molecular Weight
75.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RBL1, also known as Retinoblastoma-like protein 1, is a pivotal regulator of the cell cycle and a key player in tumor suppression. Its study is particularly significant in the context of cancer research, as mutations or dysregulation of RBL1 are implicated in various malignancies, including retinoblastoma and other types of tumors. The protein functions primarily by binding to E2F transcription factors, thereby inhibiting cell cycle progression from the G1 to the S phase, thus preventing uncontrolled cell proliferation. Recent studies have explored the use of recombinant RBL1 proteins to better understand their structure and function, aiming to elucidate the mechanisms behind its tumor-suppressive activity. Recombinant protein technology allows for the production of large quantities of pure RBL1, facilitating biochemical assays, crystallization studies, and functional analyses. These investigations are essential for developing potential therapeutic strategies that could reactivate or mimic RBL1 function in cancer cells where its activity is compromised. Furthermore, understanding the post-translational modifications and interactions of RBL1 might reveal novel pathways for targeted therapies. As such, research on recombinant RBL1 proteins is a promising frontier in the quest for innovative cancer treatments and enhancing our overall knowledge of cell cycle regulation.
