Analytical Data
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基因名
HEXB
- Application
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别名
HEXB；Beta-hexosaminidase subunit beta
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P07686
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表达区间
43-556aa
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氨基酸序列
ARAPSVSAKP GPALWPLPLS VKMTPNLLHL APENFYISHS PNSTAGPSCT LLEEAFRRYH GYIFGFYKWH HEPAEFQAKT QVQQLLVSIT LQSECDAFPN ISSDESYTLL VKEPVAVLKA NRVWGALRGL ETFSQLVYQD SYGTFTINES TIIDSPRFSH RGILIDTSRH YLPVKIILKT LDAMAFNKFN VLHWHIVDDQ SFPYQSITFP ELSNKGSYSL SHVYTPNDVR MVIEYARLRG IRVLPEFDTP GHTLSWGKGQ KDLLTPCYSR QNKLDSFGPI NPTLNTTYSF LTTFFKEISE VFPDQFIHLG GDEVEFKCWE SNPKIQDFMR QKGFGTDFKK LESFYIQKVL DIIATINKGS IVWQEVFDDK AKLAPGTIVE VWKDSAYPEE LSRVTASGFP VILSAPWYLD LISYGQDWRK YYKVEPLDFG GTQKQKQLFI GGEACLWGEY VDATNLTPRL WPRASAVGER LWSSKDVRDM DDAYDRLTRH RCRMVERGIA AQPLYAGYCN HENM
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分子量
60 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HEXB, or Hexosaminidase B, is an important lysosomal enzyme that plays a crucial role in the catabolism of glycosphingolipids, particularly GM2 gangliosides. Mutations in the HEXB gene, which encodes the HEXB enzyme, are associated with Tay-Sachs disease and Sandhoff disease, both of which are fatal lysosomal storage disorders. Research into recombinant HEXB proteins has gained significant attention due to their potential therapeutic applications. Scientists aim to produce active forms of the enzyme through recombinant DNA technology, facilitating studies on enzyme activity, substrate specificity, and pathogenic mechanisms of HEXB-related diseases. Moreover, the engineered HEXB proteins can be used to develop enzyme replacement therapies, offering hope for patients suffering from these debilitating conditions. Current studies focus on optimizing expression systems, enhancing enzyme stability, and assessing therapeutic efficacy in cellular and animal models. Understanding the structure-function relationships of HEXB and its interactions with various substrates is also critical for developing effective treatments. As a result, ongoing research on recombinant HEXB proteins plays a vital role in paving the way for innovative therapies and enhancing our understanding of lysosomal dysfunctions.
