Analytical Data
-
基因名
zot
- Application
-
别名
zot；Zona occludens toxin
-
种属
E.coli
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P38442
-
表达区间
1-399aa
-
氨基酸序列
MSIFIHHGAPGSYKTSGALWLRLLPAIKSGRHIITNVRGLNLERMAKYLKMDVSDISIEFIDTDHPDGRLTMARFWHWARKDAFLFIDECGRIWPPRLTVTNLKALDTPPDLVAEDRPESFEVAFDMHRHHGWDICLTTPNIAKVHNMIREAAEIGYRHFNRATVGLGAKFTLTTHDAANSGQMDSHALTRQVKKIPSPIFKMYASTTTGKARDTMAGTALWKDRKILFLFGMVFLMFSYSFYGLHDNPIFTGGNDATIESEQSEPQSKATVGNAVGSKAVAPASFGFCIGRLCVQDGFVTVGDERYRLVDNLDIPYRGLWATGHHIYKDTLTVFFETESGSVPTELFASSYRYKVLPLPDFNHFVVFDTFAAQALWVEVKRGLPIKTENDKKGLNSIF
-
分子量
46.9 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Zot (Zonula Occludens toxin) is a protein derived from the enterotoxin of Vibrio cholerae, known for its ability to disrupt tight junctions in epithelial cells and alter intestinal permeability. The study of Zot has gained significant attention in the context of gastrointestinal health and disease, particularly in understanding how it influences the dynamics of the intestinal barrier. Researchers have focused on its molecular mechanisms, revealing that Zot interacts with the cellular machinery to modulate signaling pathways, leading to alterations in cell-cell adhesion and paracellular transport. This has implications not only for cholera pathogenesis but also for other conditions involving compromised gut barrier function, such as inflammatory bowel disease and irritable bowel syndrome. Additionally, the potential therapeutic applications of Zot, such as in drug delivery or the modulation of inflammatory responses, are being explored. By leveraging its unique properties, scientists aim to develop strategies that could enhance intestinal health or treat gut-related disorders. Overall, the research on Zot and its recombinant forms is a promising area that bridges microbiology, immunology, and therapeutic development, highlighting the intricate relationship between microbial components and host physiology.
