Analytical Data
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Gene name
zot
- Application
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Alternative Names
zot；Zona occludens toxin
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P38442
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Expression Region
1-399aa
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AA Sequence
MSIFIHHGAPGSYKTSGALWLRLLPAIKSGRHIITNVRGLNLERMAKYLKMDVSDISIEFIDTDHPDGRLTMARFWHWARKDAFLFIDECGRIWPPRLTVTNLKALDTPPDLVAEDRPESFEVAFDMHRHHGWDICLTTPNIAKVHNMIREAAEIGYRHFNRATVGLGAKFTLTTHDAANSGQMDSHALTRQVKKIPSPIFKMYASTTTGKARDTMAGTALWKDRKILFLFGMVFLMFSYSFYGLHDNPIFTGGNDATIESEQSEPQSKATVGNAVGSKAVAPASFGFCIGRLCVQDGFVTVGDERYRLVDNLDIPYRGLWATGHHIYKDTLTVFFETESGSVPTELFASSYRYKVLPLPDFNHFVVFDTFAAQALWVEVKRGLPIKTENDKKGLNSIF
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Molecular Weight
46.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Zot (Zonula Occludens toxin) is a protein derived from the enterotoxin of Vibrio cholerae, known for its ability to disrupt tight junctions in epithelial cells and alter intestinal permeability. The study of Zot has gained significant attention in the context of gastrointestinal health and disease, particularly in understanding how it influences the dynamics of the intestinal barrier. Researchers have focused on its molecular mechanisms, revealing that Zot interacts with the cellular machinery to modulate signaling pathways, leading to alterations in cell-cell adhesion and paracellular transport. This has implications not only for cholera pathogenesis but also for other conditions involving compromised gut barrier function, such as inflammatory bowel disease and irritable bowel syndrome. Additionally, the potential therapeutic applications of Zot, such as in drug delivery or the modulation of inflammatory responses, are being explored. By leveraging its unique properties, scientists aim to develop strategies that could enhance intestinal health or treat gut-related disorders. Overall, the research on Zot and its recombinant forms is a promising area that bridges microbiology, immunology, and therapeutic development, highlighting the intricate relationship between microbial components and host physiology.
