Analytical Data
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Gene name
ACOX1
- Application
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Alternative Names
ACOX1；ACOX；Peroxisomal acyl-coenzyme A oxidase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15067
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Expression Region
1-660aa
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AA Sequence
MNPDLRRERD SASFNPELLT HILDGSPEKT RRRREIENMI LNDPDFQHED LNFLTRSQRY EVAVRKSAIM VKKMREFGIA DPDEIMWFKK LHLVNFVEPV GLNYSMFIPT LLNQGTTAQK EKWLLSSKGL QIIGTYAQTE MGHGTHLRGL ETTATYDPET QEFILNSPTV TSIKWWPGGL GKTSNHAIVL AQLITKGKCY GLHAFIVPIR EIGTHKPLPG ITVGDIGPKF GYDEIDNGYL KMDNHRIPRE NMLMKYAQVK PDGTYVKPLS NKLTYGTMVF VRSFLVGEAA RALSKACTIA IRYSAVRHQS EIKPGEPEPQ ILDFQTQQYK LFPLLATAYA FQFVGAYMKE TYHRINEGIG QGDLSELPEL HALTAGLKAF TSWTANTGIE ACRMACGGHG YSHCSGLPNI YVNFTPSCTF EGENTVMMLQ TARFLMKSYD QVHSGKLVCG MVSYLNDLPS QRIQPQQVAV WPTMVDINSP ESLTEAYKLR AARLVEIAAK NLQKEVIHRK SKEVAWNLTS VDLVRASEAH CHYVVVKLFS EKLLKIQDKA IQAVLRSLCL LYSLYGISQN AGDFLQGSIM TEPQITQVNQ RVKELLTLIR SDAVALVDAF DFQDVTLGSV LGRYDGNVYE NLFEWAKNSP LNKAEVHESY KHLKSLQSKL
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ACOX1 (acyl-CoA oxidase 1) is a crucial enzyme in the peroxisomal fatty acid beta-oxidation pathway, responsible for catalyzing the first step of the oxidation process, which converts long-chain acyl-CoA into enoyl-CoA, producing hydrogen peroxide as a byproduct. This enzyme plays a fundamental role in lipid metabolism and energy homeostasis, and its deficiency has been linked to various metabolic disorders, including Zellweger syndrome and other peroxisomal biogenesis disorders. Recent studies have highlighted the significance of ACOX1 in maintaining cellular lipid composition and regulating inflammatory responses, making it a potential therapeutic target for metabolic diseases and conditions related to oxidative stress. The recombinant expression of ACOX1 in model systems has allowed researchers to investigate its biochemical properties, substrate specificity, and regulatory mechanisms more comprehensively. Understanding the structure-function relationship of the ACOX1 protein can lead to insights into its role in human health and disease, paving the way for the development of novel strategies to combat lipid metabolism-related disorders. Furthermore, advances in protein engineering techniques, including site-directed mutagenesis and high-throughput screening, have opened new avenues for enhancing the enzyme's activity and stability, potentially leading to innovative applications in biotechnology and synthetic biology.
