Analytical Data
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Gene name
botB
- Application
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Alternative Names
botB；SYB2；Vesicle-associated membrane Protein 2
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P10844
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Expression Region
1-427aa
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AA Sequence
MPVTINNFNYNDPIDNNNIIMMEPPFARGTGRYYKAFKITDRIWIIPERYTFGYKPEDFNKSSGIFNRDVCEYYDPDYLNTNDKKNIFLQTMIKLFNRIKSKPLGEKLLEMIINGIPYLGDRRVPLEEFNTNIASVTVNKLISNPGEVERKKGIFANLIIFGPGPVLNENETIDIGIQNHFASREGFGGIMQMKFCPEYVSVFNNVQENKGASIFNRRGYFSDPALILMHELIHVLHGLYGIKVDDLPIVPNEKKFFMQSTDAIQAEELYTFGGQDPSIITPSTDKSIYDKVLQNFRGIVDRLNKVLVCISDPNININIYKNKFKDKYKFVEDSEGKYSIDVESFDKLYKSLMFGFTETNIAENYKIKTRASYFSDSLPPVKIKNLLDNEIYTIEEGFNISDKDMEKEYRGQNKAINKQAYEEISKE
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Molecular Weight
51.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of BotB, a key virulence factor produced by the bacterium *Burkholderia pseudomallei*, has garnered significant attention due to its role in the pathogenesis of melioidosis, a serious infectious disease prevalent in tropical regions. BotB is a type III secretion system effector protein that is implicated in modulating host cell signaling pathways to facilitate bacterial invasion and survival within host cells. Research on BotB focuses on understanding its structure, function, and mechanism of action, as well as its interactions with host proteins. This knowledge is critical for developing new therapeutic strategies aimed at mitigating the effects of *B. pseudomallei* infections. Additionally, the ability of BotB to evade the host immune response highlights its potential as a target for vaccine development. Investigating the recombinant form of BotB not only provides insights into its biological functions but also allows for the exploration of antigenic properties that could enhance vaccine efficacy. The combination of structural biology, immunology, and pathophysiology in BotB research aims to pave the way for improved clinical outcomes for individuals affected by melioidosis and to address the broader implications of *B. pseudomallei* as a threat to public health.
