Analytical Data
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基因名
DOT1L
- Application
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别名
DOT1L；KIAA1814；KMT4；Histone-lysine N-methyltransferase. H3 lysine-79 specific
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8TEK3
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表达区间
1-435aa
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氨基酸序列
MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPD LKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNT RPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDE IKMTDDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDR EFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVD HQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLK GSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRESK SNAATPTKGPEGKVAGPADAPMDSGAEEEKAGAATVKKPSPSKARKKKLN KKGRKMAGRKRGRPKKMNTANPERKPKKNQTALDA
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DOT1L (Disruptor of Telomeric Silencing 1 Like) is a crucial histone methyltransferase primarily recognized for its role in mediating the methylation of histone H3 at lysine 79 (H3K79). This modification is essential for regulating gene expression, chromatin structure, and DNA repair processes. The study of DOT1L has garnered significant attention due to its implications in various cancer types, particularly in the context of mixed-lineage leukemia (MLL) rearrangements, where aberrant DOT1L activity is linked to oncogenic transformations. Researchers have begun investigating DOT1L as a potential therapeutic target, aiming to disrupt its methyltransferase activity as a means to inhibit the proliferation of cancer cells. Additionally, the exploration of DOT1L's substrate specificity and its interactions with other chromatin-modifying enzymes has provided insights into the complexity of epigenetic regulation. The development of recombinant DOT1L proteins has been pivotal for these studies, enabling the dissection of its biochemical properties and facilitating high-throughput screening for small-molecule inhibitors. As such, understanding the functional dynamics of DOT1L not only enhances our comprehension of chromatin biology but also opens new avenues for targeted cancer therapies.
