Analytical Data
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Gene name
CART
- Application
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Alternative Names
CART；CART；Cocaine- and amphetamine-regulated transcript Protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16568
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Expression Region
1-116aa
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AA Sequence
MESSRVRLLP LLGAALLLML PLLGTRAQED AELQPRALDI YSAVDDASHE KELIEALQEV LKKLKSKRVP IYEKKYGQVP MCDAGEQCAV RKGARIGKLC DCPRGTSCNS FLLKCL
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Molecular Weight
36 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CART (Cocaine and amphetamine-regulated transcript) is a neuropeptide that plays a critical role in the regulation of appetite, reward, and stress response. Initial studies on CART emerged from investigations into the neurobiological effects of drugs of abuse, such as cocaine and amphetamines, which were found to significantly alter CART expression in the brain. As research progressed, it became clear that CART is not only involved in substance use but also has broader implications for neuroendocrine function and metabolic regulation. The peptide is expressed in various regions of the brain, including the hypothalamus, which is crucial for governing feeding behavior and energy homeostasis. Furthermore, CART has been implicated in mood disorders, suggesting its potential as a target for therapeutic interventions in psychiatric conditions. Recent advances in recombinant protein technology have enabled the production of CART peptides for comprehensive studies on their biological activity and mechanisms of action. Researchers are investigating the signaling pathways modulated by CART and its interactions with other neuropeptides and neurotransmitters, aiming to elucidate its role in both physiological and pathological states. Overall, the study of CART recombinant proteins is essential for understanding their multifaceted roles in the brain, contributing to our knowledge of metabolic diseases, addiction, and mood regulation, and potentially guiding the development of innovative treatment strategies.
