Analytical Data
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Gene name
RAB11FIP5
- Application
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Alternative Names
GAF 1; Gaf-1; GAF1; gamma SNAP associated factor 1; Gamma-SNAP-associated factor 1; KIAA0857; Phosphoprotein pp75; pp75; RAB11 family interacting protein 5 (class I); Rab11 family-interacting protein 5; Rab11 FIP5; Rab11 interacting protein; Rab11 interacting protein Rip11; Rab11-FIP5; Rab11-interacting protein Rip11; Rab11fip5; RFIP5_HUMAN; RIP11
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BXF6
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Expression Region
1-653 aa
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AA Sequence
MALVRGAEPA AGPSRWLPTH VQVTVLRARG LRGKSSGAGS TSDAYTVIQV GREKYSTSVV EKTHGCPEWR EECSFELPPG ALDGLLRAQE ADAGPAPWAA SSAAACELVL TTMHRSLIGV DKFLGQATVA LDEVFGAGRA QHTQWYKLHS KPGKKEKERG EIEVTIQFTR NNLSASMFDL SMKDKPRSPF SKIRDKMKGK KKYDLESASA ILPSSAIEDP DLGSLGKMGK AKGFFLRNKL RKSSLTQSNT SLGSDSTLSS ASGSLAYQGP GAELLTRSPS RSSWLSTEGG RDSAQSPKLF THKRTYSDEA NQMRVAPPRA LLDLQGHLDA ASRSSLCVNG SHIYNEEPQG PVRHRSSISG SLPSSGSLQA VSSRFSEEGP RSTDDTWPRG SRSNSSSEAV LGQEELSAQA KVLAPGASHP GEEEGARLPE GKPVQVATPI VASSEAVAEK EGARKEERKP RMGLFHHHHQ GLSRSELGRR SSLGEKGGPI LGASPHHSSS GEEKAKSSWF GLREAKDPTQ KPSPHPVKPL SAAPVEGSPD RKQSRSSLSI ALSSGLEKLK TVTSGSIQPV TQAPQAGQMV DTKRLKDSAV LDQSAKYYHL THDELISLLL QRERELSQRD EHVQELESYI DRLLVRIMET SPTLLQIPPG PPK
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Molecular Weight
70.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RAB11FIP5, a member of the RAB11 family interacting protein (FIP) family, plays a crucial role in the endocytic recycling pathway, particularly in the regulation of membrane trafficking and cellular signaling. This protein interacts specifically with the RAB11 GTPase, a key regulator of recycling endosomes, facilitating the transport of various cargoes back to the plasma membrane or to other intracellular destinations. Research into RAB11FIP5 has gained traction due to its involvement in various physiological processes and its potential link to pathological conditions, including cancer and neurodegenerative diseases. The recombinant expression and purification of RAB11FIP5 are essential for biochemical and structural studies, allowing researchers to dissect its functional mechanisms and interactions at the molecular level. Moreover, understanding the dynamics of RAB11FIP5 can offer insights into the broader implications of membrane trafficking in maintaining cellular homeostasis and contribute to the development of targeted therapeutic strategies for diseases associated with dysregulated endosomal trafficking. Studying the recombinant protein version of RAB11FIP5 can thus be pivotal for elucidating its role in cell biology and disease, making it a target of interest for both fundamental research and potential therapeutic applications.
