Analytical Data
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Gene name
MAP2K4
- Application
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Alternative Names
MAP2K4；JNKK1；MEK4；MKK4；Dual specificity mitogen-activated Protein kinase kinase 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P45985
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Expression Region
33-399aa
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AA Sequence
AVSSMQGKRKALKLNFANPPFKSTARFTLNPNPTGVQNPHIERLRTHSIE SSGKLKISPEQHWDFTAEDLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKR IRSTVDEKEQKQLLMDLDVVMRSSDCPYIVQFYGALFREGDCWICMELMS TSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLKENLKIIHRDIKP SNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQ GYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSE EREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEVACYVC KILDQMPATPSSPMYVD
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MAP2K4, also known as MKK4, is a crucial kinase in the mitogen-activated protein kinase (MAPK) signaling pathway, playing a vital role in cellular processes such as proliferation, differentiation, and stress response. As a dual-specificity kinase, MAP2K4 facilitates the activation of downstream MAPK proteins, including JUN N-terminal kinase (JNK) and p38 MAP kinase, which are involved in important physiological and pathological processes, including inflammation, apoptosis, and oncogenesis. Aberrant expression or mutation of MAP2K4 has been implicated in various cancers and inflammatory diseases, making it a potential therapeutic target. Researchers have focused on the recombinant expression of MAP2K4 to better understand its functional mechanisms and interactions within signaling networks. The production of recombinant MAP2K4 enables detailed structural and functional studies, allowing for the identification of specific substrates and the elucidation of its regulatory role in cancer progression and response to therapy. Understanding MAP2K4's mechanistic pathways is essential for developing targeted therapies that exploit its role in disease, thereby offering potential for novel intervention strategies in cancer and other related disorders.
