Analytical Data
-
Gene name
MANF
- Application
-
Alternative Names
MANF；ARMET；ARP；Mesencephalic astrocyte-derived neurotrophic factor
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P55145
-
Expression Region
25-182aa
-
AA Sequence
LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
-
Molecular Weight
18 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor) is a member of the neurotrophic factor family known for its role in protecting neurons and promoting their survival under stress conditions. Discovered in the late 1990s, MANF has garnered significant attention due to its neuroprotective properties, particularly in models of neurodegenerative diseases such as Parkinson's and Alzheimer's. Research indicates that MANF is intricately involved in the unfolded protein response (UPR), which is crucial for maintaining cellular homeostasis and function. Unlike other neurotrophic factors, MANF is primarily expressed in astrocytes and is released under conditions of cellular stress to combat neuronal cell death. Studies have shown that recombinant forms of MANF can enhance neuronal survival, promote axonal growth, and improve functional recovery in various experimental models. Additionally, investigations into its mechanism of action suggest that MANF may exert its effects through binding to specific receptors and activating signaling pathways that bolster cell resilience. As interest grows in harnessing MANF's therapeutic potential, ongoing research is focused on optimizing its production, understanding its interactions within the nervous system, and exploring its efficacy in preclinical and clinical settings for the treatment of neurodegenerative disorders. Overall, MANF represents a promising candidate for novel neuroprotective strategies, highlighting the importance of neurotrophic factors in brain health and disease management.
