Analytical Data
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Gene name
PPP3CC
- Application
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Alternative Names
PPP3CC;CALNA3;CNA3;Serine/threonine-Protein phosphatase 2B catalytic subunit gamma isoform
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P48454
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Expression Region
2-512aa
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AA Sequence
SGRRFHLSTTDRVIKAVPFPPTQRLTFKEVFENGKPKVDVLKNHLVKEGRLEEEVALKIINDGAAILRQEKTMIEVDAPITVCGDIHGQFFDLMKLFEVGGSPSNTRYLFLGDYVDRGYFSIECVLYLWSLKINHPKTLFLLRGNHECRHLTDYFTFKQECRIKYSEQVYDACMETFDCLPLAALLNQQFLCVHGGMSPEITSLDDIRKLDRFTEPPAFGPVCDLLWSDPSEDYGNEKTLEHYTHNTVRGCSYFYSYPAVCEFLQNNNLLSIIRAHEAQDAGYRMYRKSQATGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELISDDEAEGSTTVRKEIIRNKIRAIGKMARVFSILRQESESVLTLKGLTPTGTLPLGVLSGGKQTIETATVEAVEAREAIRGFSLQHKIRSFEEARGLDRINERMPPRKDSIHAGGPMKSVTSAHSHAAHRSDQGKKAHS
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Molecular Weight
62.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PPP3CC, also known as protein phosphatase 3, catalytic subunit gamma, plays a crucial role in various cellular processes by dephosphorylating serine and threonine residues on target proteins. It is a component of the protein phosphatase 2B (PP2B) complex, which is essential for calcium/calmodulin-dependent signaling pathways. Dysregulation of PPP3CC has been implicated in several diseases, including cancer, cardiovascular diseases, and neurodegenerative disorders. Research has focused on understanding its structure, function, and regulatory mechanisms to elucidate its involvement in pathological conditions and identify potential therapeutic targets. The recombinant expression of PPP3CC enables scientists to study its enzymatic activity, interaction with different substrates, and effects of various inhibitors. This research provides insights into the fundamental biology of cellular signaling and reveals the potential of PPP3CC as a biomarker or therapeutic target in disease contexts, promoting the advancement of novel treatment strategies. By investigating the precise role of PPP3CC in cellular signaling pathways, researchers aim to develop a deeper understanding of its contributions to health and disease.











